ArticleApplied and environmental microbiology2015
Biofilm-Forming Abilities of Shiga Toxin-Producing Escherichia coli Isolates Associated with Human Infections.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 60 citations in OpenAlex.
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- Lipopolysaccharide Core Truncation in InvasiveInternational journal of molecular sciences · 2024Article
- Extracellular components in enteroaggregativeFrontiers in cellular and infection microbiology · 2024Article
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- Extracellular matrix-degrading enzymes as a biofilm control strategy for food-related microorganisms.Food science and biotechnology · 2023Review
- Biofilm-forming antimicrobial-resistant pathogenicHeliyon · 2023Article
- Effectiveness of Bacteriophages against Biofilm-Forming Shiga-ToxigenicFoods (Basel, Switzerland) · 2023Article
- Protective Effect of Select Bacterial Species Representative of Fresh Produce on Human Norovirus Surrogates Exposed to Disinfecting Pulsed Light.Applied and environmental microbiology · 2023Article
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- Molecular Epidemiology of Extraintestinal PathogenicFrontiers in cellular and infection microbiology · 2021Article
- Silver Sulfadiazine Eradicates Antibiotic-TolerantJournal of clinical medicine · 2020Article
- Inactivation of Multi-Drug Resistant Non-TyphoidalPathogens (Basel, Switzerland) · 2020Article
- Article
- Antibiotic Resistance, Virulence Factors, Phenotyping, and Genotyping of Non-International journal of molecular sciences · 2020Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Forming biofilms may be a survival strategy of Shiga toxin-producing Escherichia coli to enable it to persist in the environment and the food industry. Here, we evaluate and characterize the biofilm-forming ability of 39 isolates of Shiga toxin-producing Escherichia coli isolates recovered from human infection and belonging to seropathotypes A, B, or C. The presence and/or production of biofilm factors such as curli, cellulose, autotransporter, and fimbriae were investigated. The polymeric matrix of these biofilms was analyzed by confocal microscopy and by enzymatic digestion. Cell viability and matrix integrity were examined after sanitizer treatments. Isolates of the seropathotype A (O157:H7 and O157:NM), which have the highest relative incidence of human infection, had a greater ability to form biofilms than isolates of seropathotype B or C. Seropathotype A isolates were unique in their ability to produce cellulose and poly-N-acetylglucosamine. The integrity of the biofilms was dependent on proteins. Two autotransporter genes, ehaB and espP, and two fimbrial genes, z1538 and lpf2, were identified as potential genetic determinants for biofilm formation. Interestingly, the ability of several isolates from seropathotype A to form biofilms was associated with their ability to agglutinate yeast in a mannose-independent manner. We consider this an unidentified biofilm-associated factor produced by those isolates. Treatment with sanitizers reduced the viability of Shiga toxin-producing Escherichia coli but did not completely remove the biofilm matrix. Overall, our data indicate that biofilm formation could contribute to the persistence of Shiga toxin-producing Escherichia coli and specifically seropathotype A isolates in the environment.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.