ArticleMolecular microbiology2016
Salt-sensitivity of σ(H) and Spo0A prevents sporulation of Bacillus subtilis at high osmolarity avoiding death during cellular differentiation.
Article in Molecular microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
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- Inactivation of guanylate kinase inHeliyon · 2024Article
- Metabolic rewiring enables ammonium assimilation via a non-canonical fumarate-based pathway.Microbial biotechnology · 2024Article
- Arabinose Plays an Important Role in Regulating the Growth and Sporulation ofInternational journal of molecular sciences · 2023Article
- PRO-Simat: Protein network simulation and design tool.Computational and structural biotechnology journal · 2023Article
- Review
- Review of anthrax: A disease of farm animals.Journal of advanced veterinary and animal research · 2022Review
- L-Proline Synthesis Mutants ofFrontiers in microbiology · 2022Article
- Learning from Nature: Bacterial Spores as a Target for Current Technologies in Medicine (Review).Sovremennye tekhnologii v meditsine · 2021Review
- 6S-2 RNA deletion in the undomesticatedRNA biology · 2021Article
- Salt Tolerance Mechanism and Species Identification of the Plant Rhizosphere Bacterium JYZ-SD2.Current microbiology · 2020Article
- Engineering strong and stress-responsive promoters inSynthetic and systems biotechnology · 2019Article
- Phenomics and Genomics Reveal Adaptation ofFrontiers in microbiology · 2019Article
- Identification of Differentially Expressed Genes duringFrontiers in microbiology · 2016Article
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Authors and funding
7 authors.
Funding
Abstract
The spore-forming bacterium Bacillus subtilis frequently experiences high osmolarity as a result of desiccation in the soil. The formation of a highly desiccation-resistant endospore might serve as a logical osmostress escape route when vegetative growth is no longer possible. However, sporulation efficiency drastically decreases concomitant with an increase in the external salinity. Fluorescence microscopy of sporulation-specific promoter fusions to gfp revealed that high salinity blocks entry into the sporulation pathway at a very early stage. Specifically, we show that both Spo0A- and SigH-dependent transcription are impaired. Furthermore, we demonstrate that the association of SigH with core RNA polymerase is reduced under these conditions. Suppressors that modestly increase sporulation efficiency at high salinity map to the coding region of sigH and in the regulatory region of kinA, encoding one the sensor kinases that activates Spo0A. These findings led us to discover that B. subtilis cells that overproduce KinA can bypass the salt-imposed block in sporulation. Importantly, these cells are impaired in the morphological process of engulfment and late forespore gene expression and frequently undergo lysis. Altogether our data indicate that B. subtilis blocks entry into sporulation in high-salinity environments preventing commitment to a developmental program that it cannot complete.
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