ArticleInfection and immunity2015
Characterization of New Virulence Factors Involved in the Intracellular Growth and Survival of Burkholderia pseudomallei.
Article in Infection and immunity, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 41 citations in OpenAlex.
- Discovery of a new fibronectin-binding surface protein ofFrontiers in cellular and infection microbiology · 2026Article
- Epidemiology and genetic diversity of Burkholderia pseudomallei from Riau Province, Indonesia.PLoS neglected tropical diseases · 2024Article
- Molecular mechanism of the one-component regulator RccR on bacterial metabolism and virulence.Nucleic acids research · 2024Article
- RNA interactome capture in Escherichia coli globally identifies RNA-binding proteins.Nucleic acids research · 2023Article
- Pathogenicity and virulence ofVirulence · 2022Review
- Differential Genetic Strategies of Burkholderia vietnamiensis and Paraburkholderia kururiensis for Root Colonization of Oryza sativa subsp.Applied and environmental microbiology · 2022Article
- Methodological tools to study species of the genus Burkholderia.Applied microbiology and biotechnology · 2021Review
- BipD ofMicroorganisms · 2021Review
- A Mutation Upstream of themSystems · 2020Article
- Trehalose and bacterial virulence.Virulence · 2020Review
- Burkholderia thailandensis Methylated Hydroxyalkylquinolines: Biosynthesis and Antimicrobial Activity in Cocultures.Applied and environmental microbiology · 2020Article
- Secondary metabolites from the Burkholderia pseudomallei complex: structure, ecology, and evolution.Journal of industrial microbiology & biotechnology · 2020Review
- Sulfonium Acids Loaded onto an Unusual Thiotemplate Assembly Line Construct the Cyclopropanol Warhead of a Burkholderia Virulence Factor.Angewandte Chemie (International ed. in English) · 2020Article
- In vitro passage alters virulence, immune activation and proteomic profiles of Burkholderia pseudomallei.Scientific reports · 2020Article
- Identification of genes required for the fitness ofMicrobial genomics · 2020Article
- Virulence of the emerging pathogen,Future microbiology · 2020Article
- Identification ofFrontiers in microbiology · 2020Article
- An Evolutionary Arms Race BetweenFrontiers in microbiology · 2020Review
- Prediction of Burkholderia pseudomallei DsbA substrates identifies potential virulence factors and vaccine targets.PloS one · 2020Article
- Modulating Pathogenesis with Mobile-CRISPRi.Journal of bacteriology · 2019Article
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Burkholderia pseudomallei, the causative agent of melioidosis, has complex and poorly understood extracellular and intracellular lifestyles. We used transposon-directed insertion site sequencing (TraDIS) to retrospectively analyze a transposon library that had previously been screened through a BALB/c mouse model to identify genes important for growth and survival in vivo. This allowed us to identify the insertion sites and phenotypes of negatively selected mutants that were previously overlooked due to technical constraints. All 23 unique genes identified in the original screen were confirmed by TraDIS, and an additional 105 mutants with various degrees of attenuation in vivo were identified. Five of the newly identified genes were chosen for further characterization, and clean, unmarked bpsl2248, tex, rpiR, bpsl1728, and bpss1528 deletion mutants were constructed from the wild-type strain K96243. Each of these mutants was tested in vitro and in vivo to confirm their attenuated phenotypes and investigate the nature of the attenuation. Our results confirm that we have identified new genes important to in vivo virulence with roles in different stages of B. pseudomallei pathogenesis, including extracellular and intracellular survival. Of particular interest, deletion of the transcription accessory protein Tex was shown to be highly attenuating, and the tex mutant was capable of providing protective immunity against challenge with wild-type B. pseudomallei, suggesting that the genes identified in our TraDIS screen have the potential to be investigated as live vaccine candidates.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.