ArticleProceedings of the National Academy of Sciences of the United States of America2016
Dissecting the interface between apicomplexan parasite and host cell: Insights from a divergent AMA-RON2 pair.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 44 citations in OpenAlex.
- Foliar Pine Pathogens From Different Kingdoms Share Defence-Eliciting Effector Proteins.Molecular plant pathology · 2025Article
- Eimeria: Navigating complex intestinal ecosystems.PLoS pathogens · 2024Review
- Oral vaccination with a recombinant Lactobacillus plantarum expressing the Eimeria tenella rhoptry neck 2 protein elicits protective immunity in broiler chickens infected with Eimeria tenella.Parasites & vectors · 2024Article
- A conserved protein of Babesia microti elicits partial protection against Babesia and Plasmodium infection.Parasites & vectors · 2023Article
- Invasion ofProceedings of the National Academy of Sciences of the United States of America · 2023Article
- The microneme adhesive repeat domain of MIC3 protein determined the site specificity ofFrontiers in immunology · 2023Article
- New Avenues to DesignFrontiers in immunology · 2022Article
- The malaria parasite sheddase SUB2 governs host red blood cell membrane sealing at invasion.eLife · 2020Article
- A Toxoplasma gondii patatin-like phospholipase contributes to host cell invasion.PLoS pathogens · 2020Article
- Article
- Time-Frequency Approach Applied to Finding Interaction Regions in Pathogenic Proteins.Bioinformatics and biology insights · 2019Article
- Strategies Developed byFrontiers in microbiology · 2019Review
- Genetic Analysis of Babesia Isolates from Cattle with Clinical Babesiosis in Sri Lanka.Journal of clinical microbiology · 2018Article
- RON4Scientific reports · 2017Article
- Gliding motility powers invasion and egress in Apicomplexa.Nature reviews. Microbiology · 2017Review
- The structure of Plasmodium falciparum 3D7_0606800 reveals a bi-lobed architecture that supports re-annotation as a Venus Flytrap protein.Protein science : a publication of the Protein Society · 2017Article
- Genomics of apicomplexan parasites.Critical reviews in biochemistry and molecular biology · 2017Review
- Macrophages facilitate the excystation and differentiation of Toxoplasma gondii sporozoites into tachyzoites following oocyst internalisation.Scientific reports · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 5 institutions in 4 countries.
Funding
Abstract
Plasmodium falciparum and Toxoplasma gondii are widely studied parasites in phylum Apicomplexa and the etiological agents of severe human malaria and toxoplasmosis, respectively. These intracellular pathogens have evolved a sophisticated invasion strategy that relies on delivery of proteins into the host cell, where parasite-derived rhoptry neck protein 2 (RON2) family members localize to the host outer membrane and serve as ligands for apical membrane antigen (AMA) family surface proteins displayed on the parasite. Recently, we showed that T. gondii harbors a novel AMA designated as TgAMA4 that shows extreme sequence divergence from all characterized AMA family members. Here we show that sporozoite-expressed TgAMA4 clusters in a distinct phylogenetic clade with Plasmodium merozoite apical erythrocyte-binding ligand (MAEBL) proteins and forms a high-affinity, functional complex with its coevolved partner, TgRON2L1. High-resolution crystal structures of TgAMA4 in the apo and TgRON2L1-bound forms complemented with alanine scanning mutagenesis data reveal an unexpected architecture and assembly mechanism relative to previously characterized AMA-RON2 complexes. Principally, TgAMA4 lacks both a deep surface groove and a key surface loop that have been established to govern RON2 ligand binding selectivity in other AMAs. Our study reveals a previously underappreciated level of molecular diversity at the parasite-host-cell interface and offers intriguing insight into the adaptation strategies underlying sporozoite invasion. Moreover, our data offer the potential for improved design of neutralizing therapeutics targeting a broad range of AMA-RON2 pairs and apicomplexan invasive stages.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.