ArticleProceedings of the National Academy of Sciences of the United States of America2016
Genetically targeted single-channel optical recording reveals multiple Orai1 gating states and oscillations in calcium influx.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed.
- Orai1 is required for CabioRxiv : the preprint server for biology · 2026Article
- Optical single-channel recording of CRAC channels with HaloTag and a CabioRxiv : the preprint server for biology · 2026Article
- Activation of nuclear CaCell reports · 2026Article
- Water in peripheral TM-interfaces of Orai1-channels triggers pore opening.Communications biology · 2024Article
- Photoswitching alters fluorescence readout of jGCaMP8 CaProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Proteolytically Activated CRAC Effectors through Designed Intramolecular Inhibition.ACS synthetic biology · 2022Article
- S-acylation of Orai1 regulates store-operated Ca2+ entry.Journal of cell science · 2022Article
- Orai1α, but not Orai1β, co-localizes with TRPC1 and is required for its plasma membrane location and activation in HeLa cells.Cellular and molecular life sciences : CMLS · 2022Article
- Plasma Membrane CaFunction (Oxford, England) · 2022Article
- Spatiotemporal regulation of store-operated calcium entry in cancer metastasis.Biochemical Society transactions · 2021Review
- Time-Dependent Image Restoration of Low-SNR Live-Cell CaInternational journal of molecular sciences · 2021Article
- 2-Methoxyestradiol and its derivatives inhibit store-operated CaBiochimica et biophysica acta. Molecular cell research · 2021Article
- Plasma Membrane Localized GCaMP-MS4A12 by Orai1 Co-Expression Shows Thapsigargin- and CaMolecules and cells · 2021Article
- Cardiac-Specific Deletion of Orai3 Leads to Severe Dilated Cardiomyopathy and Heart Failure in Mice.Journal of the American Heart Association · 2021Article
- Orai-vascular endothelial-cadherin signaling complex regulates high-glucose exposure-induced increased permeability of mouse aortic endothelial cells.BMJ open diabetes research & care · 2021Article
- Supra-Molecular Assemblies of ORAI1 at Rest Precede Local Accumulation into Puncta after Activation.International journal of molecular sciences · 2021Article
- Three-Dimensional Model of Sub-Plasmalemmal CaFrontiers in immunology · 2021Article
- Cell-wide mapping of Orai1 channel activity reveals functional heterogeneity in STIM1-Orai1 puncta.The Journal of general physiology · 2020Article
- STIM2 targets Orai1/STIM1 to the AKAP79 signaling complex and confers coupling of CaProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Arachidonic Acid Attenuates Cell Proliferation, Migration and Viability by a Mechanism Independent on Calcium Entry.International journal of molecular sciences · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Orai1 comprises the pore-forming subunit of the Ca(2+) release-activated Ca(2+) (CRAC) channel. When bound and activated by stromal interacting molecule 1 (STIM1), an endoplasmic reticulum (ER)-resident calcium sensor, Orai1 channels possess high selectivity for calcium but extremely small conductance that has precluded direct recording of single-channel currents. We have developed an approach to visualize Orai1 activity by fusing Orai1 to fluorescent, genetically encoded calcium indicators (GECIs). The GECI-Orai1 probes reveal local Ca(2+) influx at STIM1-Orai1 puncta. By whole cell recording, these fusions are fully functional as CRAC channels. When GECI-Orai1 and the CRAC-activating domain (CAD) of STIM1 were coexpressed at low levels and imaged using a total internal reflectance fluorescence microscope, cells exhibited sporadic fluorescence transients the size of diffraction-limited spots and the brightness of a few activated GECI proteins. Transients typically rose rapidly and fell into two classes according to duration: briefer "flickers" lasting only a few hundred milliseconds, and longer "pulses" lasting one to several seconds. The size, intensity, trace shape, frequency, distribution, physiological characteristics, and association with CAD binding together demonstrate that GECI-Orai1 fluorescence transients correspond to single-channel Orai1 responses. Single Orai1 channels gated by CAD, and small Orai1 puncta gated by STIM1, exhibit repetitive fluctuations in single-channel output. CAD binding supports a role in open state maintenance and reveals a second phase of CAD/STIM1 binding after channel opening. These first recordings of single-channel Orai1 currents reveal unexpected dynamics, and when paired with CAD association, support multiple single-channel states.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.