ArticleProceedings of the National Academy of Sciences of the United States of America2016
Pan-neuronal imaging in roaming Caenorhabditis elegans.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 135 papers.
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Who cites it
135 citing papers in PubMed.
- Article
- The dual interpretation of edge time series: Time-varying connectivity versus statistical interaction.iScience · 2026Article
- Mechanisms of surface and volume light scattering fromiScience · 2026Article
- Deconstructing a behavioral state: parallel neural integrators control distinct features of an aversive behavioral state inbioRxiv : the preprint server for biology · 2026Article
- Article
- Neural sequences underlying directed turning in Caenorhabditis elegans.Nature neuroscience · 2026Article
- A modular multi-color fluorescence microscope for simultaneous tracking of cellular activity and behavior.Nature communications · 2026Article
- An epifluorescence microscope design for naturalistic behavior and cellular activity in freely moving Caenorhabditis elegans.Nature communications · 2026Article
- Intrinsic Diversity in Odor-Evoked Calcium Rises acrossThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Deep neural networks to register and annotate cells in moving and deforming nervous systems.eLife · 2026Article
- Efficient pheromone navigation via antagonistic detectors in Caenorhabditis elegans male.Nature communications · 2026Article
- CeDNe: A multi-scale computational framework for modeling structure-function relationships in thebioRxiv : the preprint server for biology · 2025Article
- Network modularity reveals context and state-dependent reorganization of time-varying functional connectivity in single-cell resolved neural activity recordings.bioRxiv : the preprint server for biology · 2025Article
- Integrative functional optical imaging approaches: Optics, microfluidics, and machine learning for neuroscience in organoids and small-animal models.Biophysical journal · 2025Review
- Bridging the gap between the connectome and whole-brain activity inbioRxiv : the preprint server for biology · 2025Article
- Article
- Parameters that influence bipartite reporter system expression in Caenorhabditis elegans.Genetics · 2025Article
- Unifying community whole-brain imaging datasets enables robust neuron identification and reveals determinants of neuron position in C. elegans.Cell reports methods · 2025Article
- Review
- CRASH2p: Closed-loop Two Photon Imaging in a Freely Moving Animal.bioRxiv : the preprint server for biology · 2024Article
75 more citing papers are in PubMed but not listed here.
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15 authors.
Funding
Abstract
We present an imaging system for pan-neuronal recording in crawling Caenorhabditis elegans. A spinning disk confocal microscope, modified for automated tracking of the C. elegans head ganglia, simultaneously records the activity and position of ∼80 neurons that coexpress cytoplasmic calcium indicator GCaMP6s and nuclear localized red fluorescent protein at 10 volumes per second. We developed a behavioral analysis algorithm that maps the movements of the head ganglia to the animal's posture and locomotion. Image registration and analysis software automatically assigns an index to each nucleus and calculates the corresponding calcium signal. Neurons with highly stereotyped positions can be associated with unique indexes and subsequently identified using an atlas of the worm nervous system. To test our system, we analyzed the brainwide activity patterns of moving worms subjected to thermosensory inputs. We demonstrate that our setup is able to uncover representations of sensory input and motor output of individual neurons from brainwide dynamics. Our imaging setup and analysis pipeline should facilitate mapping circuits for sensory to motor transformation in transparent behaving animals such as C. elegans and Drosophila larva.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.