ArticleMolecular and cellular biology2015
Arsenic Directly Binds to and Activates the Yeast AP-1-Like Transcription Factor Yap8.
Article in Molecular and cellular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 43 citations in OpenAlex.
- Arsenic-sensing domain controls ACR3 transporter trafficking in Marchantia polymorpha.The Plant cell · 2026Article
- Arsenic binds to nuclear transport factors and disrupts nucleocytoplasmic transport.Journal of cell science · 2025Article
- Direct binding of arsenicals to nuclear transport factors disrupts nucleocytoplasmic transport.bioRxiv : the preprint server for biology · 2025Article
- Multilevel Regulation of Membrane Proteins in Response to Metal and Metalloid Stress: A Lesson from Yeast.International journal of molecular sciences · 2024Review
- Mechanisms of genotoxicity and proteotoxicity induced by the metalloids arsenic and antimony.Cellular and molecular life sciences : CMLS · 2023Review
- Review
- Resistance to Arsenite and Arsenate inInternational journal of environmental research and public health · 2022Article
- Identification of novel arsenic resistance genes in yeast.MicrobiologyOpen · 2022Article
- Etp1 confers arsenite resistance by affecting ACR3 expression.FEMS yeast research · 2022Article
- Differential metabolism of arsenicals regulates Fps1-mediated arsenite transport.The Journal of cell biology · 2022Article
- Arsenic perception and signaling: The yet unexplored world.Frontiers in plant science · 2022Review
- Genome-wide imaging screen uncovers molecular determinants of arsenite-induced protein aggregation and toxicity.Journal of cell science · 2021Article
- Inorganic arsenic induces sex-dependent pathological hypertrophy in the heart.American journal of physiology. Heart and circulatory physiology · 2021Article
- The ancillary N-terminal region of the yeast AP-1 transcription factor Yap8 contributes to its DNA binding specificity.Nucleic acids research · 2020Article
- Thiol-based direct threat sensing by the stress-activated protein kinase Hog1.Science signaling · 2019Article
- Methylated metabolite of arsenite blocks glycerol production in yeast by inhibition of glycerol-3-phosphate dehydrogenase.Molecular biology of the cell · 2019Article
- Yeast AP-1 like transcription factors (Yap) and stress response: a current overview.Microbial cell (Graz, Austria) · 2019Review
- Article
- Intracellular mechanism by which arsenite activates the yeast stress MAPK Hog1.Molecular biology of the cell · 2018Article
- AP-1 (bZIP) Transcription Factors as Potential Regulators of Metallothionein Gene Expression inFrontiers in genetics · 2018Article
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
The AP-1-like transcription factor Yap8 is critical for arsenic tolerance in the yeast Saccharomyces cerevisiae. However, the mechanism by which Yap8 senses the presence of arsenic and activates transcription of detoxification genes is unknown. Here we demonstrate that Yap8 directly binds to trivalent arsenite [As(III)] in vitro and in vivo and that approximately one As(III) molecule is bound per molecule of Yap8. As(III) is coordinated by three sulfur atoms in purified Yap8, and our genetic and biochemical data identify the cysteine residues that form the binding site as Cys132, Cys137, and Cys274. As(III) binding by Yap8 does not require an additional yeast protein, and Yap8 is regulated neither at the level of localization nor at the level of DNA binding. Instead, our data are consistent with a model in which a DNA-bound form of Yap8 acts directly as an As(III) sensor. Binding of As(III) to Yap8 triggers a conformational change that in turn brings about a transcriptional response. Thus, As(III) binding to Yap8 acts as a molecular switch that converts inactive Yap8 into an active transcriptional regulator. This is the first report to demonstrate how a eukaryotic protein couples arsenic sensing to transcriptional activation.
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