ArticleMolecular cell2016
A Dicer-Independent Route for Biogenesis of siRNAs that Direct DNA Methylation in Arabidopsis.
Article in Molecular cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
73 citing papers in PubMed, 146 citations in OpenAlex.
- Presence and function of small RNAs during plant reproduction.RNA biology · 2026Review
- RNAi in the Rhizarian Phytopathogen Plasmodiophora brassicae: The Causal Agent of Clubroot Disease in Cruciferous Crops.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- RNA regulation in plants.Science China. Life sciences · 2026Review
- Advances in Plant Antiviral RNAi: From Host DCLs/RDRs to Diversified Viral Counteracting Strategies.Viruses · 2026Review
- Epigenetic gene regulation in plants and its potential applications in crop improvement.Nature reviews. Molecular cell biology · 2025Review
- RNA interference and turnover in plants -a complex partnership.Frontiers in plant science · 2025Review
- Genome-wide identification, characterization and expression analysis of key gene families in RNA silencing in centipedegrass.BMC genomics · 2024Article
- An RdDM-independent function of Pol V transcripts in gene regulation and plant defence.Nature plants · 2024Article
- Recent Advances in Studies of Genomic DNA Methylation and Its Involvement in Regulating Drought Stress Response in Crops.Plants (Basel, Switzerland) · 2024Review
- The plant siRNA landscape.The Plant cell · 2024Review
- A Non-Canonical Pathway Induced by Externally Applied Virus-Specific dsRNA in Potato Plants.International journal of molecular sciences · 2023Article
- Cotranscriptional RNA processing and modification in plants.The Plant cell · 2023Review
- The DNA methylation landscape of the root-knot nematode-induced pseudo-organ, the gall, in Arabidopsis, is dynamic, contrasting over time, and critically important for successful parasitism.The New phytologist · 2022Article
- Roles of RNA silencing in viral and non-viral plant immunity and in the crosstalk between disease resistance systems.Nature reviews. Molecular cell biology · 2022Review
- RNA Pol IV induces antagonistic parent-of-origin effects on Arabidopsis endosperm.PLoS biology · 2022Article
- CRISPR-Cas-mediated transcriptional control and epi-mutagenesis.Plant physiology · 2022Review
- Reinforcement of CHH methylation through RNA-directed DNA methylation ensures sexual reproduction in rice.Plant physiology · 2022Article
- CRISPR/Cas9-mediated knockout of the DCL2 and DCL4 genes in Nicotiana benthamiana and its productivity of recombinant proteins.Plant cell reports · 2022Article
- Article
- You shall not pass! A Chromatin barrier story in plants.Frontiers in plant science · 2022Review
13 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
DNA methylation directed by 24-nucleotide (nt) small interfering RNAs (siRNAs) plays critical roles in gene regulation and transposon silencing in Arabidopsis. 24-nt siRNAs are known to be processed from double-stranded RNAs by Dicer-like 3 (DCL3) and loaded into the effector Argonaute 4 (AGO4). Here we report a distinct class of siRNAs independent of DCLs (sidRNAs). sidRNAs are present as ladders of ∼ 20-60 nt in length, often having the same 5' ends but differing in 3' ends by 1-nt steps. We further show that sidRNAs are associated with AGO4 and capable of directing DNA methylation. Finally we show that sidRNA production depends on distributive 3'-5' exonucleases. Our findings suggest an alternative route for siRNA biogenesis. Precursor transcripts are bound by AGO4 and subsequently subjected to 3'-5' exonucleolytic trimming for maturation. We propose that sidRNAs generated through this route are the initial triggers of de novo DNA methylation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.