ArticleThe Journal of biological chemistry2016
Biochemical Composition and Assembly of Biosilica-associated Insoluble Organic Matrices from the Diatom Thalassiosira pseudonana.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 82 citations in OpenAlex.
- The Ratio of Silicon to Chitin in Diatom Cell Walls Influences Their Resilience to Environmental Stress.Research (Washington, D.C.) · 2026Article
- The Role of Confinement in Biomineralization.Chemical reviews · 2025Review
- Integrative Multi-Omics Analyses Reveal the Global Regulation Network of the MicroalgaBiology · 2025Article
- Harnessing Microalgae as Sustainable Cell Factories for Polyamine-Based Nanosilica for Biomedical Applications.Molecules (Basel, Switzerland) · 2025Review
- Silica-Biomacromolecule Interactions: Toward a Mechanistic Understanding of Silicification.Biomacromolecules · 2025Review
- Intracellular morphogenesis of diatom silica is guided by local variations in membrane curvature.Nature communications · 2024Article
- Enhancement of hemostatic properties of Cyclotella cryptica frustule through genetic manipulation.Biotechnology for biofuels and bioproducts · 2023Article
- Genome analysis of Parmales, the sister group of diatoms, reveals the evolutionary specialization of diatoms from phago-mixotrophs to photoautotrophs.Communications biology · 2023Article
- The molecular basis for pore pattern morphogenesis in diatom silica.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Targeting motifs in frustule-associated proteins from the centric diatomFrontiers in plant science · 2022Article
- Structural evidence for extracellular silica formation by diatoms.Nature communications · 2021Article
- In-silico Exploration of Channel Type and Efflux Silicon Transporters and Silicification Proteins in 80 Sequenced Viridiplantae Genomes.Plants (Basel, Switzerland) · 2020Article
- Comparative Gene Analysis Focused on Silica Cell Wall Formation: Identification of Diatom-Specific SET Domain Protein Methyltransferases.Marine biotechnology (New York, N.Y.) · 2020Article
- Article
- Integration of proteome and transcriptome refines key molecular processes underlying oil production inBiotechnology for biofuels · 2020Article
- Characterization of a New Protein Family Associated With the Silica Deposition Vesicle Membrane Enables Genetic Manipulation of Diatom Silica.Scientific reports · 2017Article
- The evolution of diatoms and their biogeochemical functions.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Silicanin-1 is a conserved diatom membrane protein involved in silica biomineralization.BMC biology · 2017Article
- Dynamic responses to silicon in Thalasiossira pseudonana - Identification, characterisation and classification of signature genes and their corresponding protein motifs.Scientific reports · 2017Article
- Biogenic and Synthetic Peptides with Oppositely Charged Amino Acids as Binding Sites for Mineralization.Materials (Basel, Switzerland) · 2017Article
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nano- and micropatterned biosilica cell walls of diatoms are remarkable examples of biological morphogenesis and possess highly interesting material properties. Only recently has it been demonstrated that biosilica-associated organic structures with specific nanopatterns (termed insoluble organic matrices) are general components of diatom biosilica. The model diatom Thalassiosira pseudonana contains three types of insoluble organic matrices: chitin meshworks, organic microrings, and organic microplates, the latter being described in the present study for the first time. To date, little is known about the molecular composition, intracellular assembly, and biological functions of organic matrices. Here we have performed structural and functional analyses of the organic microrings and organic microplates from T. pseudonana. Proteomics analysis yielded seven proteins of unknown function (termed SiMat proteins) together with five known silica biomineralization proteins (four cingulins and one silaffin). The location of SiMat1-GFP in the insoluble organic microrings and the similarity of tyrosine- and lysine-rich functional domains identifies this protein as a new member of the cingulin protein family. Mass spectrometric analysis indicates that most of the lysine residues of cingulins and the other insoluble organic matrix proteins are post-translationally modified by short polyamine groups, which are known to enhance the silica formation activity of proteins. Studies with recombinant cingulins (rCinY2 and rCinW2) demonstrate that acidic conditions (pH 5.5) trigger the assembly of mixed cingulin aggregates that have silica formation activity. Our results suggest an important role for cingulins in the biogenesis of organic microrings and support the hypothesis that this type of insoluble organic matrix functions in biosilica morphogenesis.
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