ArticlePloS one2015
Identification of Thyroid Hormones and Functional Characterization of Thyroid Hormone Receptor in the Pacific Oyster Crassostrea gigas Provide Insight into Evolution of the Thyroid Hormone System.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 68 citations in OpenAlex.
- Mapping molluscan endocrinology: a systematic and critical appraisal.Biological reviews of the Cambridge Philosophical Society · 2026Pooled it
- Transcriptomic and Ultrastructural Analyses Reveal the Mechanisms of Accelerated Depuration Induced byAnimals : an open access journal from MDPI · 2026Article
- Substrate for Thyroid Hormone Synthesis: Biochemistry, Evolution, and Physiology.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Review
- Comparative Transcriptomics Provides Insight into the Neuroendocrine Regulation of Spawning in the Black-Lip Rock Oyster (International journal of molecular sciences · 2025Article
- Thyroid hormones reversibly inhibit metamorphic development in ophiuroid larvae.The Journal of experimental biology · 2025Article
- Shared regulatory function of non-genomic thyroid hormone signaling in echinoderm skeletogenesis.EvoDevo · 2024Article
- Transcriptional regulation analysis reveals the complexity of metamorphosis in the Pacific oyster (Marine life science & technology · 2023Article
- Article
- Thyroid hormone membrane receptor binding and transcriptional regulation in the sea urchinFrontiers in endocrinology · 2023Article
- Thyroid-like hormone signaling in invertebrates and its potential role in initial screening of thyroid hormone system disrupting chemicals.Integrated environmental assessment and management · 2023Review
- Regulation of Tyrosinase Gene Expression by Retinoic Acid Pathway in the Pacific OysterInternational journal of molecular sciences · 2022Article
- Thyroid Hormones in Hemolymph of Red King Crabs from the Barents Sea.Animals : an open access journal from MDPI · 2022Article
- Larval metamorphosis is inhibited by methimazole and propylthiouracil that reveals possible hormonal action in the mussel Mytilus coruscus.Scientific reports · 2021Article
- Review
- Function and Evolution of Nuclear Receptors in Environmental-Dependent Postembryonic Development.Frontiers in cell and developmental biology · 2021Review
- Proteome of larval metamorphosis induced by epinephrine in the Fujian oyster Crassostrea angulata.BMC genomics · 2020Article
- Thyroid Hormone Signalling: From the Dawn of Life to the Bedside.Journal of molecular evolution · 2020Review
- Thyroid hormone receptor localization in target tissues.The Journal of endocrinology · 2018Review
- Thyroid Hormones Accelerate Initiation of Skeletogenesis via MAPK (ERK1/2) in Larval Sea Urchins (Frontiers in endocrinology · 2018Article
- Evolutionarily conserved TRH neuropeptide pathway regulates growth inProceedings of the National Academy of Sciences of the United States of America · 2017Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Thyroid hormones (THs) play important roles in development, metamorphosis, and metabolism in vertebrates. During the past century, TH functions were regarded as a synapomorphy of vertebrates. More recently, accumulating evidence has gradually convinced us that TH functions also occur in invertebrate chordates. To date, however, TH-related studies in non-chordate invertebrates have been limited. In this study, THs were qualitatively detected by two reliable methods (HPLC and LC/MS) in a well-studied molluscan species, the Pacific oyster Crassostrea gigas. Quantitative measurement of THs during the development of C. gigas showed high TH contents during embryogenesis and that oyster embryos may synthesize THs endogenously. As a first step in elucidating the TH signaling cascade, an ortholog of vertebrate TH receptor (TR), the most critical gene mediating TH effects, was cloned in C. gigas. The sequence of CgTR has conserved DNA-binding and ligand-binding domains that normally characterize these receptors. Experimental results demonstrated that CgTR can repress gene expression through binding to promoters of target genes and can interact with oyster retinoid X receptor. Moreover, CgTR mRNA expression was activated by T4 and the transcriptional activity of CgTR promoter was repressed by unliganded CgTR protein. An atypical thyroid hormone response element (CgDR5) was found in the promoter of CgTR, which was verified by electrophoretic mobility shift assay (EMSA). These results indicated that some of the CgTR function is conserved. However, the EMSA assay showed that DNA binding specificity of CgTR was different from that of the vertebrate TR and experiments with two dual-luciferase reporter systems indicated that l-thyroxine, 3,3',5-triiodothyronine, and triiodothyroacetic acid failed to activate the transcriptional activity of CgTR. This is the first study to functionally characterize TR in mollusks. The presence of THs and the functions of CgTR in mollusks contribute to better understanding of the evolution of the TH system.
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