ArticleJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research2016
Coupling Effect of Water and Proteoglycans on the In Situ Toughness of Bone.
Article in Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 40 citations in OpenAlex.
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- Intra-Articular Mesenchymal Stem Cell Injection for Knee Osteoarthritis: Mechanisms and Clinical Evidence.International journal of molecular sciences · 2022Review
- Cartilage Oligomeric Matrix Protein, Diseases, and Therapeutic Opportunities.International journal of molecular sciences · 2022Review
- Bone hydration: How we can evaluate it, what can it tell us, and is it an effective therapeutic target?Bone reports · 2022Article
- Stability and remineralization of proteoglycan-infused dentin substrate.Dental materials : official publication of the Academy of Dental Materials · 2021Article
- Removal of glycosaminoglycans affects the in situ mechanical behavior of extrafibrillar matrix in bone.Journal of the mechanical behavior of biomedical materials · 2021Article
- The Collagen Origin Influences the Degradation Kinetics of Guided Bone Regeneration Membranes.Polymers · 2021Article
- Small leucine-rich proteoglycans in physiological and biomechanical function of bone.Matrix biology plus · 2021Review
- Characterization of Microstructural Changes on Biglycan Induced Mice Bone by Low-Field Nuclear Magnetic Resonance.Applied physics (Kowloon, China) · 2021Article
- Molecular origin of viscoelasticity in mineralized collagen fibrils.Biomaterials science · 2021Article
- Biglycan and chondroitin sulfate play pivotal roles in bone toughness via retaining bound water in bone mineral matrix.Matrix biology : journal of the International Society for Matrix Biology · 2020Article
- Engineering a Corneal Stromal Equivalent Using a Novel Multilayered Fabrication Assembly Technique.Tissue engineering. Part A · 2020Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Proteoglycans (PGs) are one type of noncollagenous proteins in the extracellular matrix of bone that primarily contain a core protein and glycosaminoglycans (GAGs). GAGs are highly polar and negatively charged, thus have a strong tendency to attract water molecules into the matrix. We hypothesized in this study that PGs in bone play a pivotal role in sustaining the toughness of the tissue only when water is present. To test the hypothesis, we used a novel nanoscratch test to measure the in situ toughness of human cadaveric bone treated with and without peptide-N-glycosidase F (PNGase F), an enzyme that specifically removes the N-linked oligosaccharides of GAGs from core proteins. Cortical bone specimens were prepared from the posterior aspect of mid-diaphyseal femurs of six (n = 6) male human donors between 51.5 ± 5.17 years old. Biochemical and histochemical assays were used to verify whether N-linked oligosaccharides were removed from bone matrix by PNGase F. By testing wet and dehydrated bone specimens, the coupling effect between water and PGs on the in situ toughness of bone was investigated. The two-way ANOVA analyses showed that removal of GAGs had significant effects on the in situ toughness of wet bone samples. In contrast, the removal of GAGs did not show significant effects on the toughness of dry bone. The results of this study, for the first time, suggest that GAGs play a pivotal role in the in situ toughness of bone only when water is present, and vice versa, water functions as a plasticizer in bone only when GAGs are present. © 2015 American Society for Bone and Mineral Research.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.