Evidence map›Paper›PMID 26709923›Full record

ArticlePloS one2015

In Situ Analysis of a Silver Nanoparticle-Precipitating Shewanella Biofilm by Surface Enhanced Confocal Raman Microscopy.

Gal Schkolnik, Matthias Schmidt, Marco G Mazza, Falk Harnisch, Niculina Musat

Erratum issuedAbstract read
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Form and function in biological filaments: a physicist's review.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2025
    Review
  2. Shewanella oneidensis: Biotechnological Application of Metal-Reducing Bacteria.Advances in biochemical engineering/biotechnology · 2025
    Review
  3. Article
  4. Hybrid sideways/longitudinal swimming in the monoflagellateJournal of the Royal Society, Interface · 2020
    Article
  5. Article
  6. Article
  7. Review on SERS of Bacteria.Biosensors · 2017
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Gal SchkolnikDepartment of Dynamics of Complex Fluids, Max Planck Institute for Dynamics and Self-Organization, Göttingen, Germany.
Matthias SchmidtDepartment of Isotope Biogeochemistry, Helmholtz Centre for Environmental Research, Leipzig, Germany.
Marco G MazzaDepartment of Dynamics of Complex Fluids, Max Planck Institute for Dynamics and Self-Organization, Göttingen, Germany.
Falk HarnischDepartment of Environmental Microbiology, Helmholtz Centre for Environmental Research, Leipzig, Germany.
Niculina MusatDepartment of Isotope Biogeochemistry, Helmholtz Centre for Environmental Research, Leipzig, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Shewanella oneidensis MR-1 is an electroactive bacterium, capable of reducing extracellular insoluble electron acceptors, making it important for both nutrient cycling in nature and microbial electrochemical technologies, such as microbial fuel cells and microbial electrosynthesis. When allowed to anaerobically colonize an Ag/AgCl solid interface, S. oneidensis has precipitated silver nanoparticles (AgNp), thus providing the means for a surface enhanced confocal Raman microscopy (SECRaM) investigation of its biofilm. The result is the in-situ chemical mapping of the biofilm as it developed over time, where the distribution of cytochromes, reduced and oxidized flavins, polysaccharides and phosphate in the undisturbed biofilm is monitored. Utilizing AgNp bio-produced by the bacteria colonizing the Ag/AgCl interface, we could perform SECRaM while avoiding the use of a patterned or roughened support or the introduction of noble metal salts and reducing agents. This new method will allow a spatially and temporally resolved chemical investigation not only of Shewanella biofilms at an insoluble electron acceptor, but also of other noble metal nanoparticle-precipitating bacteria in laboratory cultures or in complex microbial communities in their natural habitats.

Indexed as

BiofilmsChemical PrecipitationCytochromesFlavinsMetal NanoparticlesMicroscopy, ConfocalMicroscopy, Electron, ScanningOxidation-ReductionPhosphatesPolysaccharides, BacterialShewanellaSilverSpectrum Analysis, RamanSurface PropertiesCytochromesFlavinsPhosphatesPolysaccharides, BacterialSilver

Identifiers

PMID26709923
PMCPMC4692441

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.