ArticleeLife2015
Observation of long-range tertiary interactions during ligand binding by the TPP riboswitch aptamer.
Article in eLife, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- Nascent RNA Folding and RNP Assembly Revealed by Single-molecule Microscopy.Journal of molecular biology · 2026Review
- Nexus between RNA conformational dynamics and functional versatility.Current opinion in structural biology · 2024Review
- Looking at Biomolecular Interactions through the Lens of Correlated Fluorescence Microscopy and Optical Tweezers.International journal of molecular sciences · 2023Review
- Single Molecule FRET Analysis of CRISPR Cas9 Single Guide RNA Folding Dynamics.The journal of physical chemistry. B · 2023Article
- Out-of-Equilibrium Biophysical Chemistry: The Case for Multidimensional, Integrated Single-Molecule Approaches.The journal of physical chemistry. B · 2021Article
- Optical tweezers in single-molecule biophysics.Nature reviews. Methods primers · 2021Article
- Intrinsic Regulatory Role of RNA Structural Arrangement in Alternative Splicing Control.International journal of molecular sciences · 2020Review
- Transcriptional Riboswitches Integrate Timescales for Bacterial Gene Expression Control.Frontiers in molecular biosciences · 2020Review
- Fluorescence-based investigations of RNA-small molecule interactions.Methods (San Diego, Calif.) · 2019Article
- Article
- Unraveling RNA dynamical behavior of TPP riboswitches: a comparison between Escherichia coli and Arabidopsis thaliana.Scientific reports · 2019Article
- Bio-Molecular Applications of Recent Developments in Optical Tweezers.Biomolecules · 2019Review
- Self-cleavage of theProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Ligand Modulates Cross-Coupling between Riboswitch Folding and Transcriptional Pausing.Molecular cell · 2018Article
- Life under the Microscope: Single-Molecule Fluorescence Highlights the RNA World.Chemical reviews · 2018Review
- Multimodal Measurements of Single-Molecule Dynamics Using FluoRBT.Biophysical journal · 2018Article
- High-Resolution Optical Tweezers Combined With Single-Molecule Confocal Microscopy.Methods in enzymology · 2017Article
- The light side of the force.eLife · 2016Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The thiamine pyrophosphate (TPP) riboswitch is a cis-regulatory element in mRNA that modifies gene expression in response to TPP concentration. Its specificity is dependent upon conformational changes that take place within its aptamer domain. Here, the role of tertiary interactions in ligand binding was studied at the single-molecule level by combined force spectroscopy and Förster resonance energy transfer (smFRET), using an optical trap equipped for simultaneous smFRET. The 'Force-FRET' approach directly probes secondary and tertiary structural changes during folding, including events associated with binding. Concurrent transitions observed in smFRET signals and RNA extension revealed differences in helix-arm orientation between two previously-identified ligand-binding states that had been undetectable by spectroscopy alone. Our results show that the weaker binding state is able to bind to TPP, but is unable to form a tertiary docking interaction that completes the binding process. Long-range tertiary interactions stabilize global riboswitch structure and confer increased ligand specificity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.