ArticlePloS one2015
Bacterial Profiling Reveals Novel "Ca. Neoehrlichia", Ehrlichia, and Anaplasma Species in Australian Human-Biting Ticks.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 62 citations in OpenAlex.
- New insights about human tick infestation features: a systematic review and meta-analysis.Environmental science and pollution research international · 2021Pooled it
- Ticks and Tick-Borne Microorganisms in Australian Wildlife: A Scoping One Health Evidence Synthesis of Reported Associations and Knowledge Gaps.Pathogens (Basel, Switzerland) · 2026Review
- Article
- Geographic Variation in the Prevalence of Candidatus Neoehrlichia procyonis in Raccoons (Procyon lotor) in the United States and Canada.MicrobiologyOpen · 2025Article
- Article
- Neoehrlichiosis in Symptomatic Immunocompetent Child, South Africa.Emerging infectious diseases · 2023Article
- The Troublesome Ticks Research Protocol: Developing a Comprehensive, Multidiscipline Research Plan for Investigating Human Tick-Associated Disease in Australia.Pathogens (Basel, Switzerland) · 2022Article
- Metatranscriptomic profiling reveals diverse tick-borne bacteria, protozoans and viruses in ticks and wildlife from Australia.Transboundary and emerging diseases · 2022Article
- The bacterial biome of ticks and their wildlife hosts at the urban-wildland interface.Microbial genomics · 2021Article
- Complete Mitochondrial Genome Sequence of the Gulf Coast Tick (Amblyomma maculatum).Microbiology resource announcements · 2021Article
- Data analysis workflow for the detection of canine vector-borne pathogens using 16 S rRNA Next-Generation Sequencing.BMC veterinary research · 2021Article
- The Efficacy of Ultrasonic Pest Repellent Devices against the Australian Paralysis Tick,Insects · 2021Article
- Parasites of wombats (family Vombatidae), with a focus on ticks and tick-borne pathogens.Parasitology research · 2021Review
- Morphological identification of ticks and molecular detection of tick-borne pathogens from bare-nosed wombats (Vombatus ursinus).Parasites & vectors · 2021Article
- Unbiased Characterization of the Microbiome and Virome of Questing Ticks.Frontiers in microbiology · 2021Article
- Illuminating the bacterial microbiome of Australian ticks withCurrent research in parasitology & vector-borne diseases · 2021Article
- Screening forPathogens (Basel, Switzerland) · 2020Article
- General Microbiota of the Soft TickBiology · 2020Article
- A novelPathogens and global health · 2020Article
- Biotic Factors Influence Microbiota of Nymph Ticks from Vegetation in Sydney, Australia.Pathogens (Basel, Switzerland) · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Australia, a conclusive aetiology of Lyme disease-like illness in human patients remains elusive, despite growing numbers of people presenting with symptoms attributed to tick bites. In the present study, we surveyed the microbial communities harboured by human-biting ticks from across Australia to identify bacteria that may contribute to this syndrome. Universal PCR primers were used to amplify the V1-2 hyper-variable region of bacterial 16S rRNA genes in DNA samples from individual Ixodes holocyclus (n = 279), Amblyomma triguttatum (n = 167), Haemaphysalis bancrofti (n = 7), and H. longicornis (n = 7) ticks. The 16S amplicons were sequenced on the Illumina MiSeq platform and analysed in USEARCH, QIIME, and BLAST to assign genus and species-level taxonomies. Nested PCR and Sanger sequencing were used to confirm the NGS data and further analyse novel findings. All 460 ticks were negative for Borrelia spp. by both NGS and nested PCR analysis. Two novel "Candidatus Neoehrlichia" spp. were identified in 12.9% of I. holocyclus ticks. A novel Anaplasma sp. was identified in 1.8% of A. triguttatum ticks, and a novel Ehrlichia sp. was identified in both A. triguttatum (1.2%) ticks and a single I. holocyclus (0.6%) tick. Further phylogenetic analysis of novel "Ca. Neoehrlichia", Anaplasma and Ehrlichia based on 1,265 bp 16S rRNA gene sequences suggests that these are new species. Determining whether these newly discovered organisms cause disease in humans and animals, like closely related bacteria do abroad, is of public health importance and requires further investigation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.