ArticleJournal of visualized experiments : JoVE2015
Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Robust identification of cell-cell communication heterogeneity in single cells.bioRxiv : the preprint server for biology · 2026Article
- Spatial and Single-Cell Transcriptomics Reveal Keratinocytes as Key Players in Vulvar Lichen Sclerosus Pathogenesis.The Journal of investigative dermatology · 2026Article
- Lineage memory shapes viral resistance barriers in human skin.bioRxiv : the preprint server for biology · 2025Article
- Diversity of human skin three-dimensional organotypic cultures.Current opinion in genetics & development · 2024Review
- Article
- Single-cell transcriptomics of human-skin-equivalent organoids.Cell reports · 2023Article
- CDK12 Is Necessary to Promote Epidermal Differentiation Through Transcription Elongation.Stem cells (Dayton, Ohio) · 2022Article
- Regulation of integrin and extracellular matrix genes by HNRNPL is necessary for epidermal renewal.PLoS biology · 2021Article
- ELL Facilitates RNA Polymerase II-Mediated Transcription of Human Epidermal Proliferation Genes.The Journal of investigative dermatology · 2021Article
- SPT6 promotes epidermal differentiation and blockade of an intestinal-like phenotype through control of transcriptional elongation.Nature communications · 2021Article
- RAS-mediated suppression of PAR3 and its effects on SCC initiation and tissue architecture occur independently of hyperplasia.Journal of cell science · 2020Article
- Single cell transcriptomics of human epidermis identifies basal stem cell transition states.Nature communications · 2020Article
- Article
- HNRNPK maintains epidermal progenitor function through transcription of proliferation genes and degrading differentiation promoting mRNAs.Nature communications · 2019Article
- Induction of hair follicle neogenesis with cultured mouse dermal papilla cells in de novo regenerated skin tissues.Journal of tissue engineering and regenerative medicine · 2019Article
- The parathyroid hormone family member TIP39 interacts with sarco/endoplasmic reticulum CaExperimental dermatology · 2017Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Organotypic cultures allow the reconstitution of a 3D environment critical for cell-cell contact and cell-matrix interactions which mimics the function and physiology of their in vivo tissue counterparts. This is exemplified by organotypic skin cultures which faithfully recapitulates the epidermal differentiation and stratification program. Primary human epidermal keratinocytes are genetically manipulable through retroviruses where genes can be easily overexpressed or knocked down. These genetically modified keratinocytes can then be used to regenerate human epidermis in organotypic skin cultures providing a powerful model to study genetic pathways impacting epidermal growth, differentiation, and disease progression. The protocols presented here describe methods to prepare devitalized human dermis as well as to genetically manipulate primary human keratinocytes in order to generate organotypic skin cultures. Regenerated human skin can be used in downstream applications such as gene expression profiling, immunostaining, and chromatin immunoprecipitations followed by high throughput sequencing. Thus, generation of these genetically modified organotypic skin cultures will allow the determination of genes that are critical for maintaining skin homeostasis.
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