ArticleVaccine2016
Development of a candidate reference material for adventitious virus detection in vaccine and biologicals manufacturing by deep sequencing.
Article in Vaccine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- Enhancing Clinical Utility: Utilization of International Standards and Guidelines for Metagenomic Sequencing in Infectious Disease Diagnosis.International journal of molecular sciences · 2024Review
- LABRADOR-A Computational Workflow for Virus Detection in High-Throughput Sequencing Data.Viruses · 2021Article
- Refinement of microbiota analysis of specimens from patients with respiratory infections using next-generation sequencing.Scientific reports · 2021Article
- High-throughput Sequencing in Vaccine Research.Journal of veterinary research · 2021Article
- Implementation of next-generation sequencing for virus identification in veterinary diagnostic laboratories.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2021Article
- Deep Impact of Random Amplification and Library Construction Methods on Viral Metagenomics Results.Viruses · 2021Article
- Report of the second international conference on next generation sequencing for adventitious virus detection in biologics for humans and animals.Biologicals : journal of the International Association of Biological Standardization · 2020Article
- Report of the 2019 NIST-FDA workshop on standards for next generation sequencing detection of viral adventitious agents in biologics and biomanufacturing.Biologicals : journal of the International Association of Biological Standardization · 2020Article
- Sensitivity and breadth of detection of high-throughput sequencing for adventitious virus detection.NPJ vaccines · 2020Article
- Next-generation sequencing of dsRNA is greatly improved by treatment with the inexpensive denaturing reagent DMSO.Microbial genomics · 2019Article
- Metagenomic analysis of viruses in toilet waste from long distance flights-A new procedure for global infectious disease surveillance.PloS one · 2019Article
- Article
- Considerations for Optimization of High-Throughput Sequencing Bioinformatics Pipelines for Virus Detection.Viruses · 2018Article
- Oxford Screening CSF and Respiratory samples ('OSCAR'): results of a pilot study to screen clinical samples from a diagnostic microbiology laboratory for viruses using Illumina next generation sequencing.BMC research notes · 2018Article
- Selection and evaluation of an efficient method for the recovery of viral nucleic acids from complex biologicals [corrected].NPJ vaccines · 2018Article
- Viral Surveillance in Serum Samples From Patients With Acute Liver Failure By Metagenomic Next-Generation Sequencing.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2017Article
- Optimization and validation of sample preparation for metagenomic sequencing of viruses in clinical samples.Microbiome · 2017Article
- Efficient and unbiased metagenomic recovery of RNA virus genomes from human plasma samples.Scientific reports · 2017Article
- Generic Amplicon Deep Sequencing to DetermineFrontiers in microbiology · 2017Article
- Evaluation of Methods for the Concentration and Extraction of Viruses from Sewage in the Context of Metagenomic Sequencing.PloS one · 2017Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
backgroundUnbiased deep sequencing offers the potential for improved adventitious virus screening in vaccines and biotherapeutics. Successful implementation of such assays will require appropriate control materials to confirm assay performance and sensitivity.
methodsA common reference material containing 25 target viruses was produced and 16 laboratories were invited to process it using their preferred adventitious virus detection assay.
resultsFifteen laboratories returned results, obtained using a wide range of wet-lab and informatics methods. Six of 25 target viruses were detected by all laboratories, with the remaining viruses detected by 4-14 laboratories. Six non-target viruses were detected by three or more laboratories.
conclusionThe study demonstrated that a wide range of methods are currently used for adventitious virus detection screening in biological products by deep sequencing and that they can yield significantly different results. This underscores the need for common reference materials to ensure satisfactory assay performance and enable comparisons between laboratories.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.