ArticleJournal of visualized experiments : JoVE2015
A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 41 citations in OpenAlex.
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- Unveiling centromeric retrotransposon dynamics through a high-quality rye genome assembly.Nature communications · 2026Article
- Comparative oligo-FISH validates genome assemblies and delivers a precise karyotype for Lens Mill. species.PloS one · 2026Article
- Preparing high-quality chromosome spreads from Crocus species for karyotyping and FISH.Molecular cytogenetics · 2025Article
- A chromosome-scale reference genome of grasspea (Lathyrus sativus).Scientific data · 2024Article
- A Flow Cytometry-Based Assessment of the Genomic Size and Ploidy Level of WildPlants (Basel, Switzerland) · 2023Article
- Prospects of telomere-to-telomere assembly in barley: Analysis of sequence gaps in the MorexV3 reference genome.Plant biotechnology journal · 2022Article
- Initial Description of the Genome ofFrontiers in plant science · 2022Article
- The Evolutionary Dynamics of Repetitive DNA and Its Impact on the Genome Diversification in the GenusFrontiers in plant science · 2021Article
- Different from tracheophytes, liverworts commonly have mixed 35S and 5S arrays.Annals of botany · 2020Article
- Fluorescence in situ hybridization in plants: recent developments and future applications.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2019Review
- Cytogenetic and molecular characteristics of rye genome in octoploid triticale (×Comparative cytogenetics · 2019Article
- Cytogenetic comparison of heteromorphic and homomorphic sex chromosomes in Coccinia (Cucurbitaceae) points to sex chromosome turnover.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2017Article
- Fluorescent labelling of in situ hybridisation probes through the copper-catalysed azide-alkyne cycloaddition reaction.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2016Article
- Fragile sites of 45S rDNA of Lolium multiflorum are not hotspots for chromosomal breakages induced by X-ray.Molecular biology reports · 2016Article
- Collinearity of homoeologous group 3 chromosomes in the genus Hordeum and Secale cereale as revealed by 3H-derived FISH analysis.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2016Article
- Application of a modified drop method for high-resolution pachytene chromosome spreads in two Phalaenopsis species.Molecular cytogenetics · 2016Article
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Preparation of chromosome spreads is a prerequisite for the successful performance of fluorescence in situ hybridization (FISH). Preparation of high quality plant chromosome spreads is challenging due to the rigid cell wall. One of the approved methods for the preparation of plant chromosomes is a so-called drop preparation, also known as drop-spreading or air-drying technique. Here, we present a protocol for the fast preparation of mitotic chromosome spreads suitable for the FISH detection of single and high copy DNA probes. This method is an improved variant of the air-dry drop method performed under a relative humidity of 50%-55%. This protocol comprises a reduced number of washing steps making its application easy, efficient and reproducible. Obvious benefits of this approach are well-spread, undamaged and numerous metaphase chromosomes serving as a perfect prerequisite for successful FISH analysis. Using this protocol we obtained high-quality chromosome spreads and reproducible FISH results for Hordeum vulgare, H. bulbosum, H. marinum, H. murinum, H. pubiflorum and Secale cereale.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.