ArticleThe Journal of cell biology1989
Formation and movement of myosin-containing structures in living fibroblasts.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 52 citations in OpenAlex.
- Arp2/3 complex is essential for actin network treadmilling as well as for targeting of capping protein and cofilin.Molecular biology of the cell · 2013Article
- Identification and characterization of Myosin from rat testicular peritubular myoid cells.Biology of reproduction · 2008Article
- Retrograde flow and myosin II activity within the leading cell edge deliver F-actin to the lamella to seed the formation of graded polarity actomyosin II filament bundles in migrating fibroblasts.Molecular biology of the cell · 2008Article
- Regulation of myosin II dynamics by phosphorylation and dephosphorylation of its light chain in epithelial cells.Molecular biology of the cell · 2007Article
- The role of myosin II motor activity in distributing myosin asymmetrically and coupling protrusive activity to cell translocation.Molecular biology of the cell · 2006Article
- Structural characterization of weakly attached cross-bridges in the A*M*ATP state in permeabilized rabbit psoas muscle.Biophysical journal · 2002Article
- Distinct roles of frontal and rear cell-substrate adhesions in fibroblast migration.Molecular biology of the cell · 2001Article
- Keratocytes generate traction forces in two phases.Molecular biology of the cell · 1999Article
- Stresses at the cell-to-substrate interface during locomotion of fibroblasts.Biophysical journal · 1999Article
- Cell crawling: first the motor, now the transmission.The Journal of cell biology · 1998Review
- Analysis of the actin-myosin II system in fish epidermal keratocytes: mechanism of cell body translocation.The Journal of cell biology · 1997Article
- Regulation of cell motility by mitogen-activated protein kinase.The Journal of cell biology · 1997Article
- Myosin II filament assemblies in the active lamella of fibroblasts: their morphogenesis and role in the formation of actin filament bundles.The Journal of cell biology · 1995Article
- Fluorescent actin analogs with a high affinity for profilin in vitro exhibit an enhanced gradient of assembly in living cells.The Journal of cell biology · 1994Article
- Non-sarcomeric mode of myosin II organization in the fibroblast lamellum.The Journal of cell biology · 1993Article
- Localization and dynamics of nonfilamentous actin in cultured cells.The Journal of cell biology · 1993Article
- Gradients in the concentration and assembly of myosin II in living fibroblasts during locomotion and fiber transport.Molecular biology of the cell · 1993Article
- Actin aggregation and embryonic epidermal wound healing.Journal of mathematical biology · 1993Article
- Microinjection of nonmuscle and smooth muscle caldesmon into fibroblasts and muscle cells.The Journal of cell biology · 1990Article
- Mechanism of the formation of contractile ring in dividing cultured animal cells. II. Cortical movement of microinjected actin filaments.The Journal of cell biology · 1990Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Gizzard myosin, fluorescently labeled with tetramethylrhodamine iodoacetamide, was microinjected into living 3T3 fibroblasts to label myosin-containing structures. The fluorophore was located predominantly on the heavy chain near the COOH terminus of the S1 head and on the 17-kD light chain. After microinjection of a tracer amount into living 3T3 cells, the fluorescent myosin showed a distribution identical to that revealed by immunofluorescence with antimyosin antibodies. Injected myosin became localized in small beads, which were found along large stress fibers, along fine fibers, and in a poorly organized form near the lamellipodia. De novo assembly of beads was observed continuously within or near the lamellipodia, suggesting that myosin molecules may undergo a constant cycling between polymerized and unpolymerized states. The nascent structures then moved away from lamellipodia and became organized into linear arrays. Similar movement was also observed for beads already associated with linear structures, and may represent a continuous flux of myosin structures. The dynamic reorganization of myosin may play an important role in cell movement and polarity.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.