ArticleJournal of visualized experiments : JoVE2015
A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 15 citations in OpenAlex.
- Aged differentiated cells reverse into native stemness-like state by niche cytokines to sustain lifelong homeostasis and tissue repair.Nature communications · 2026Article
- miR-184 represses β-catenin and behaves as a skin tumor suppressor.Cell death & disease · 2024Article
- Corneal regeneration: insights in epithelial stem cell heterogeneity and dynamics.Current opinion in genetics & development · 2022Review
- Evidence for a phenotypic switch in corneal afferents after lacrimal gland excision.Experimental eye research · 2022Article
- CRISPR/Cas9-mediated knock-in strategy at the Rosa26 locus in cattle fetal fibroblasts.PloS one · 2022Article
- Discrete limbal epithelial stem cell populations mediate corneal homeostasis and wound healing.Cell stem cell · 2021Article
- Two-photon live imaging of single corneal stem cells reveals compartmentalized organization of the limbal niche.Cell stem cell · 2021Article
- Limbal stem cell diseases.Experimental eye research · 2021Review
- Corneal Epithelial Abrasion with Ocular Burr As a Model for Cornea Wound Healing.Journal of visualized experiments : JoVE · 2018Article
- Overview of the reporter genes and reporter mouse models.Animal models and experimental medicine · 2018Review
- Study of corneal epithelial progenitor origin and the Yap1 requirement using keratin 12 lineage tracing transgenic mice.Scientific reports · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lineage tracing experiments define the origin, fate and behavior of cells in a specific tissue or organism. This technique has been successfully applied for many decades, revealing seminal findings in developmental biology. More recently, it was adopted by stem cell biologists to identify and track different stem cell populations with minimal experimental intervention. The recent developments in mouse genetics, the availability of a large number of mouse strains, and the advancements in fluorescent microscopy allow the straightforward design of powerful lineage tracing systems for various tissues with basic expertise, using commercially available tools. We have recently taken advantage of this powerful methodology to explore the origin and fate of stem cells at the ocular surface using R26R-Confetti mouse. This model offers a multi-color genetic system, for the expression of 4 fluorescent genes in a random manner. Here we describe the principles of this methodology and provide an adaptable protocol for designing lineage tracing experiments; specifically for the corneal epithelium as well as for other tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.