ArticleBiotechnology for biofuels2015
Biomass accessibility analysis using electron tomography.
Article in Biotechnology for biofuels, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Determination of hydroxyl groups in biorefinery resources via quantitativeNature protocols · 2019Article
- Multimodal analysis of pretreated biomass species highlights generic markers of lignocellulose recalcitrance.Biotechnology for biofuels · 2018Article
- Dynamical assessment of fluorescent probes mobility in poplar cell walls reveals nanopores govern saccharification.Biotechnology for biofuels · 2018Article
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Authors and funding
5 authors.
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Abstract
backgroundSubstrate accessibility to catalysts has been a dominant theme in theories of biomass deconstruction. However, current methods of quantifying accessibility do not elucidate mechanisms for increased accessibility due to changes in microstructure following pretreatment.
resultsWe introduce methods for characterization of surface accessibility based on fine-scale microstructure of the plant cell wall as revealed by 3D electron tomography. These methods comprise a general framework, enabling analysis of image-based cell wall architecture using a flexible model of accessibility. We analyze corn stover cell walls, both native and after undergoing dilute acid pretreatment with and without a steam explosion process, as well as AFEX pretreatment.
conclusionImage-based measures provide useful information about how much pretreatments are able to increase biomass surface accessibility to a wide range of catalyst sizes. We find a strong dependence on probe size when measuring surface accessibility, with a substantial decrease in biomass surface accessibility to probe sizes above 5-10 nm radius compared to smaller probes.
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