ArticleEngineering (Beijing, China)2015
Smartphone-Imaged HIV-1 Reverse-Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) on a Chip from Whole Blood.
Article in Engineering (Beijing, China), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed.
- A smartphone-based particle diffusometry platform for sub-attomolar detection of Vibrio cholerae in environmental water.Biosensors & bioelectronics · 2020Trial
- Rapid simultaneous self-testing of HIV and HCV viral loads with integrated RNA extraction and multiplex RT-PCR in under 1 hour.Biosensors & bioelectronics · 2025Article
- Compact multiplex PCR device for HIV-1 and HIV-2 viral load determination from finger-prick whole blood in resource-limited settings.Biosensors & bioelectronics · 2025Article
- A handheld HIV detection platform using paper-based sample preparation and real-time isothermal amplification.Microsystems & nanoengineering · 2024Article
- Real-Time Visualization of HIV-1 RNA Detection Using Loop-Mediated Isothermal Amplification-Enabled Particle Diffusometry.ACS sensors · 2024Article
- Sample preparation and detection methods in point-of-care devices towards future at-home testing.Lab on a chip · 2024Review
- Large-volume RT-LAMP enables extraction-free amplification of HIV RNA from fingerstick plasma.Analytica chimica acta · 2024Article
- Development of a LAMP-Based Diagnostic for the Detection of Multiple HIV-1 Strains.Biosensors · 2024Article
- Quantitative mRNA expression measurement at home.Scientific reports · 2024Article
- Specific Detection of RHDV GI.1 and GI.2 by RT-LAMP-CRISPR/Cas12a Platform.Transboundary and emerging diseases · 2024Article
- Developing a surface acoustic wave-induced microfluidic cell lysis device for point-of-care DNA amplification.Engineering in life sciences · 2024Article
- Compact Point-of-Care Device for Self-Administered HIV Viral Load Tests from Whole Blood.ACS sensors · 2023Article
- Review of HIV Self Testing Technologies and Promising Approaches for the Next Generation.Biosensors · 2023Review
- Human-centered design of a smartphone-based self-test for HIV viral load monitoring.Journal of clinical and translational science · 2023Article
- Microfluidic Devices for HIV Diagnosis and Monitoring at Point-of-Care (POC) Settings.Biosensors · 2022Review
- Smartphone clip-on instrument and microfluidic processor for rapid sample-to-answer detection of Zika virus in whole blood using spatial RT-LAMP.The Analyst · 2022Article
- Fingerpick Blood-Based Nucleic Acid Testing on A USB Interfaced Device towards HIV self-testing.Biosensors & bioelectronics · 2022Article
- Quantitative isothermal amplification on paper membranes using amplification nucleation site analysis.Lab on a chip · 2022Article
- Portable real-time colorimetric LAMP-device for rapid quantitative detection of nucleic acids in crude samples.Scientific reports · 2022Article
- Bridging the gap between development of point-of-care nucleic acid testing and patient care for sexually transmitted infections.Lab on a chip · 2022Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Viral load measurements are an essential tool for the long-term clinical care of hum an immunodeficiency virus (HIV)-positive individuals. The gold standards in viral load instrumentation, however, are still too limited by their size, cost, and sophisticated operation for these measurements to be ubiquitous in remote settings with poor healthcare infrastructure, including parts of the world that are disproportionately affected by HIV infection. The challenge of developing a point-of-care platform capable of making viral load more accessible has been frequently approached but no solution has yet emerged that meets the practical requirements of low cost, portability, and ease-of-use. In this paper, we perform reverse-transcription loop-mediated isothermal amplification (RT-LAMP) on minimally processed HIV-spiked whole blood samples with a microfluidic and silicon microchip platform, and perform fluorescence measurements with a consumer smartphone. Our integrated assay shows amplification from as few as three viruses in a ~ 60 nL RT-LAMP droplet, corresponding to a whole blood concentration of 670 viruses per µL of whole blood. The technology contains greater power in a digital RT-LAMP approach that could be scaled up for the determination of viral load from a finger prick of blood in the clinical care of HIV-positive individuals. We demonstrate that all aspects of this viral load approach, from a drop of blood to imaging the RT-LAMP reaction, are compatible with lab-on-a-chip components and mobile instrumentation.
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