ArticlePlant physiology2016
TOPP4 Regulates the Stability of PHYTOCHROME INTERACTING FACTOR5 during Photomorphogenesis in Arabidopsis.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 44 citations in OpenAlex.
- The protein phosphatase OsPP1a dephosphorylates and stabilizes CatC to scavenge excess HPlant communications · 2026Article
- Genome-wide analysis of sunflower (Helianthus annuus) and Orobanche cumana reveals HaEXLA2 and HaEXLB2 as negative regulators of lignin-based resistance.Plant cell reports · 2026Article
- Genomic Insights into Adaptation ofPlants (Basel, Switzerland) · 2026Article
- GhTOPP4aD and GhRAF36 inversely regulate cotton (Gossypium hirsutum) response to ABA and salt stress through reversible phosphorylation of GhABI1.Plant biotechnology journal · 2025Article
- Type One Protein Phosphatase 4aD Negatively Regulates Cotton (International journal of molecular sciences · 2025Article
- PHOSPHATASE 2A dephosphorylates PHYTOCHROME-INTERACTING FACTOR3 to modulate photomorphogenesis in Arabidopsis.The Plant cell · 2024Article
- What is going on inside of phytochrome B photobodies?The Plant cell · 2024Review
- Arabidopsis NF-YC7 Interacts with CRY2 and PIF4/5 to Repress Blue Light-Inhibited Hypocotyl Elongation.International journal of molecular sciences · 2023Article
- The role of forkhead-associated (FHA)-domain proteins in plant biology.Plant molecular biology · 2023Review
- ABA signaling prevents phosphodegradation of the SR45 splicing factor to alleviate inhibition of early seedling development in Arabidopsis.Plant communications · 2023Article
- Type one protein phosphatase regulates fixed-carbon starvation-induced autophagy in Arabidopsis.The Plant cell · 2022Article
- Molecular and biochemical characterization of two 4-coumarate: CoA ligase genes in tea plant (Camellia sinensis).Plant molecular biology · 2022Article
- Rice protein phosphatase 1 regulatory subunits OsINH2 and OsINH3 participate actively in growth and adaptive responses under abscisic acid.Frontiers in plant science · 2022Article
- Integration of Light and Brassinosteroid Signaling during Seedling Establishment.International journal of molecular sciences · 2021Review
- Wheat Type One Protein Phosphatase Participates in the Brassinosteroid Control of Root Growth via Activation of BES1.International journal of molecular sciences · 2021Article
- Regulation of DELLA Proteins by Post-translational Modifications.Plant & cell physiology · 2020Review
- Role of Protein Phosphatase1 Regulatory Subunit3 in Mediating the Abscisic Acid Response.Plant physiology · 2020Article
- SAUR15 Promotes Lateral and Adventitious Root Development via Activating HPlant physiology · 2020Article
- Article
- UVR8 disrupts stabilisation of PIF5 by COP1 to inhibit plant stem elongation in sunlight.Nature communications · 2019Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In plants, photoreceptors transfer light signals to phytochrome-interacting factors (PIFs), inducing the rapid phosphorylation and degradation of PIFs to promote photomorphogenesis. However, the phosphatase responsible for PIF dephosphorylation remains unknown. In this study, we identified a type 1 protein phosphatase, TOPP4, that is essential for PIF5 protein stability in Arabidopsis (Arabidopsis thaliana). Compared with the wild type, the dominant-negative mutant, topp4-1, displayed reduced hypocotyl length and larger apical hook and cotyledon opening angle under red light. Overexpression of topp4-1 in the wild type led to defects that were similar to those in the topp4-1 mutant. Red light induced phytochrome B (phyB)-dependent TOPP4 expression in hypocotyls. The topp4-1 mutation weakened the closed cotyledon angle of phyB-9 and phyA-211 phyB-9, while overexpression of TOPP4 significantly repressed the short hypocotyls of phyB-green fluorescent protein seedlings, indicating that TOPP4 and phyB function in an antagonistic way during photomorphogenesis. Protein interaction assays and phosphorylation studies demonstrate that TOPP4 interacts directly with PIF5 and dephosphorylates it. Furthermore, TOPP4 inhibits the red light-induced ubiquitination and degradation of PIF5. These findings demonstrate that dephosphorylation of PIF5 by TOPP4 inhibits its ubiquitin-mediated degradation during photomorphogenesis. These data outline a novel phytochrome signaling mechanism by which TOPP4-mediated dephosphorylation of PIF5 attenuates phytochrome-dependent light responses.
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