ArticleInternational journal of molecular sciences2015
Stable Expression of Basic Fibroblast Growth Factor in Chloroplasts of Tobacco.
Article in International journal of molecular sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 39 citations in OpenAlex.
- Developing downstream processes for the purification of recombinant proteins and small molecules from Nicotiana benthamiana biomass.Plant biotechnology journal · 2026Review
- Recent trends and advances in chloroplast engineering and transformation methods.Frontiers in plant science · 2025Review
- Exploring recent progress of molecular farming for therapeutic and recombinant molecules in plant systems.Heliyon · 2024Review
- Biopotentials of Collagen Scaffold Impregnated with Plant-Cell-Derived Epidermal Growth Factor in Defective Bone Healing.Materials (Basel, Switzerland) · 2023Article
- Efficient expression of fusion human epidermal growth factor in tobacco chloroplasts.BMC biotechnology · 2023Article
- High-level transient production of a protease-resistant mutant form of human basic fibroblast growth factor inPlant biotechnology (Tokyo, Japan) · 2022Article
- Production of Recombinant Active Human TGFβ1 inFrontiers in plant science · 2022Article
- Plant Molecular Farming: A Viable Platform for Recombinant Biopharmaceutical Production.Plants (Basel, Switzerland) · 2020Review
- Local supplementation with plant-derived recombinant human FGF2 protein enhances bone formation in critical-sized calvarial defects.Journal of bone and mineral metabolism · 2019Article
- Evaluation of lettuce chloroplast and soybean cotyledon as platforms for production of functional bone morphogenetic protein 2.Transgenic research · 2019Article
- Expression of VGRNb-PE immunotoxin in transplastomic lettuce (Lactuca sativa L.).Plant molecular biology · 2018Article
- Preface-Plant Proteomic Research.International journal of molecular sciences · 2017Article
- Recent achievements obtained by chloroplast transformation.Plant methods · 2017Review
- Codon Optimization to Enhance Expression Yields Insights into Chloroplast Translation.Plant physiology · 2016Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Basic fibroblast growth factor (bFGF) is a multifunctional factor in acceleration of cell proliferation, differentiation and transference, and therefore widely used in clinical applications. In this study, expression vector pWX-Nt03 harboring a codon-optimized bFGF gene was constructed and introduced into the tobacco chloroplasts by particle bombardment. After four rounds of selection, bFGF was proved to integrate into the chloroplast genome of regenerated plants and two of four transgenic plants were confirmed to be homoplastomic by PCR and Southern hybridization. ELISA assay indicated that bFGF represented approximately 0.1% of total soluble protein in the leaves of transplastomic tobacco plants. This is the first report of bFGF expression via chloroplast transformation in model plant, providing an additional option for the production of chloroplast-produced therapeutic proteins.
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