ArticleJournal of neuroscience methods2016
A fully automated Drosophila olfactory classical conditioning and testing system for behavioral learning and memory assessment.
Article in Journal of neuroscience methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Individuality across environmental context ineLife · 2026Article
- Elevated serotonin receptor 2A signaling restores learning and memory in a Fragile X syndrome model.Scientific reports · 2026Article
- Behavioral and genetic correlates of heterogeneity in learning performance in individual honeybees, Apis mellifera.PloS one · 2024Article
- Dysregulation of BMP, Wnt, and Insulin Signaling in Fragile X Syndrome.Frontiers in cell and developmental biology · 2022Review
- Multi-camera field monitoring reveals costs of learning for parasitoid foraging behaviour.The Journal of animal ecology · 2021Article
- Behavior Individuality: A Focus onFrontiers in physiology · 2021Review
- Active Inferants: An Active Inference Framework for Ant Colony Behavior.Frontiers in behavioral neuroscience · 2021Article
- Ethoscopes: An open platform for high-throughput ethomics.PLoS biology · 2017Article
- Logical fallacies in animal model research.Behavioral and brain functions : BBF · 2017Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
backgroundAversive olfactory classical conditioning has been the standard method to assess Drosophila learning and memory behavior for decades, yet training and testing are conducted manually under exceedingly labor-intensive conditions. To overcome this severe limitation, a fully automated, inexpensive system has been developed, which allows accurate and efficient Pavlovian associative learning/memory analyses for high-throughput pharmacological and genetic studies. NEW
methodThe automated system employs a linear actuator coupled to an odorant T-maze with airflow-mediated transfer of animals between training and testing stages. Odorant, airflow and electrical shock delivery are automatically administered and monitored during training trials. Control software allows operator-input variables to define parameters of Drosophila learning, short-term memory and long-term memory assays.
resultsThe approach allows accurate learning/memory determinations with operational fail-safes. Automated learning indices (immediately post-training) and memory indices (after 24h) are comparable to traditional manual experiments, while minimizing experimenter involvement. COMPARISON WITH EXISTING
methodsThe automated system provides vast improvements over labor-intensive manual approaches with no experimenter involvement required during either training or testing phases. It provides quality control tracking of airflow rates, odorant delivery and electrical shock treatments, and an expanded platform for high-throughput studies of combinational drug tests and genetic screens. The design uses inexpensive hardware and software for a total cost of ∼$500US, making it affordable to a wide range of investigators.
conclusionsThis study demonstrates the design, construction and testing of a fully automated Drosophila olfactory classical association apparatus to provide low-labor, high-fidelity, quality-monitored, high-throughput and inexpensive learning and memory behavioral assays.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.