ArticlePlant cell reports2016
Activation of anthocyanin biosynthesis by expression of the radish R2R3-MYB transcription factor gene RsMYB1.
Article in Plant cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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46 citing papers in PubMed, 80 citations in OpenAlex.
- Article
- Long non-coding RNA RsLNC2361 positively regulate anthocyanin accumulation in radish.BMC plant biology · 2026Article
- Fine-Mapping of a Red-Skinned Taproot Gene in Radish (Plants (Basel, Switzerland) · 2025Article
- Evolutionary trajectory of transcription factors and selection of targets for metabolic engineering.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Sequence and epigenetic variations of R2R3-MYB transcription factors determine the diversity of taproot skin and flesh colors in different cultivated types of radish (Raphanus sativus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Molecular Regulatory Network of Anthocyanin Accumulation in Black Radish Skin as Revealed by Transcriptome and Metabonome Analysis.International journal of molecular sciences · 2023Article
- Gap-free genome assembly and comparative analysis reveal the evolution and anthocyanin accumulation mechanism ofHorticulture research · 2023Article
- Effect of structural variation in the promoter region ofFrontiers in plant science · 2023Article
- The long noncoding RNAFrontiers in plant science · 2023Article
- Agronomic Behavior of Mexican Roselle Cultivars Produced under Protected Agricultural Conditions.Plants (Basel, Switzerland) · 2022Article
- RsTTG1, a WD40 Protein, Interacts with the bHLH Transcription Factor RsTT8 to Regulate Anthocyanin and Proanthocyanidin Biosynthesis inInternational journal of molecular sciences · 2022Article
- Anthocyanin Biosynthesis Induced by MYB Transcription Factors in Plants.International journal of molecular sciences · 2022Review
- Transcriptional regulation of proanthocyanidin biosynthesis pathway genes and transcription factors in Indigofera stachyodes Lindl. roots.BMC plant biology · 2022Article
- Article
- Metabolic and transcriptome analysis of dark red taproot in radish (Raphanus sativus L.).PloS one · 2022Article
- Loss of the R2R3 MYB Transcription Factor RsMYB1 Shapes Anthocyanin Biosynthesis and Accumulation inInternational journal of molecular sciences · 2021Article
- Intron-retained radish (Raphanus sativus L.) RsMYB1 transcripts found in colored-taproot lines enhance anthocyanin accumulation in transgenic Arabidopsis plants.Plant cell reports · 2021Article
- Anthocyanin Biosynthesis Genes as Model Genes for Genome Editing in Plants.International journal of molecular sciences · 2021Review
- Virus-induced gene silencing (VIGS) analysis shows involvement of the LsSTPK gene in lettuce (Plant signaling & behavior · 2021Article
- Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageRsMYB1, a MYB TF of red radish origin, was characterized as a positive regulator to transcriptionally activate the anthocyanin biosynthetic machinery by itself in Arabidopsis and tobacco plants. Anthocyanins, providing the bright red-orange to blue-violet colors, are flavonoid-derived pigments with strong antioxidant activity that have benefits for human health. We isolated RsMYB1, which encodes an R2R3-MYB transcription factor (TF), from red radish plants (Raphanus sativus L.) that accumulate high levels of anthocyanins. RsMYB1 shows higher expression in red radish than in common white radish, in both leaves and roots, at different growth stages. Consistent with RsMYB1 function as an anthocyanin-promoting TF, red radishes showed higher expression of all six anthocyanin biosynthetic and two anthocyanin regulatory genes. Transient expression of RsMYB1 in tobacco showed that RsMYB1 is a positive regulator of anthocyanin production with better efficiency than the basic helix-loop-helix (bHLH) TF gene B-Peru. Also, the synergistic effect of RsMYB1 with B-Peru was larger than the effect of the MYB TF gene mPAP1D with B-peru. Arabidopsis plants stably expressing RsMYB1 produced red pigmentation throughout the plant, accompanied by up-regulation of the six structural and two regulatory genes for anthocyanin production. This broad transcriptional activation of anthocyanin biosynthetic machinery in Arabidopsis included up-regulation of TRANSPARENT TESTA8, which encodes a bHLH TF. These results suggest that overexpression of RsMYB1 promotes anthocyanin production by triggering the expression of endogenous bHLH genes as potential binding partners for RsMYB1. In addition, RsMYB1-overexpressing Arabidopsis plants had a higher antioxidant capacity than did non-transgenic control plants. Taken together, RsMYB1 is an actively positive regulator for anthocyanins biosynthesis in radish plants and it might be one of the best targets for anthocyanin production by single gene manipulation being applicable in diverse plant species.
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