ReviewAmerican journal of physiology. Cell physiology2016
Beyond Ussing's chambers: contemporary thoughts on integration of transepithelial transport.
Review in American journal of physiology. Cell physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 47 citations in OpenAlex.
- Mapping Intestinal Paracellular Perm Eability in Mice: Regional and Cellular Variability Under Physiological and Stimulated Conditions.FASEB bioAdvances · 2026Article
- Larazotide Acetate Protects the Intestinal Mucosal Barrier from Anoxia/Reoxygenation Injury via Various Cellular Mechanisms.Biomedicines · 2025Article
- Lipopolysaccharide transport during long-chain fatty acid exposure is mediated by caveolin-1 dependent endocytosis in murine jejunum.Tissue barriers · 2025Article
- Intestinal Membrane Function in Inflammatory Bowel Disease.Pharmaceutics · 2023Review
- Gut-on-a-Chip Research for Drug Development: Implications of Chip Design on Preclinical Oral Bioavailability or Intestinal Disease Studies.Biomimetics (Basel, Switzerland) · 2023Review
- Electrophysiological Measurements of Isolated Blood Vessels.Bio-protocol · 2022Article
- Shenling Baizhu Powder () Ameliorates Pi (Spleen)-Deficiency-Induced Functional Diarrhea in Rats.Chinese journal of integrative medicine · 2021Article
- Use of Ussing Chambers to Measure Paracellular Permeability to Macromolecules in Mouse Intestine.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Ussing Chamber Methods to Study the Esophageal Epithelial Barrier.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Enlightening the frontiers of neurogastroenterology through optogenetics.American journal of physiology. Gastrointestinal and liver physiology · 2020Review
- The Regulation of Intestinal Mucosal Barrier by Myosin Light Chain Kinase/Rho Kinases.International journal of molecular sciences · 2020Review
- The "Leaky Gut": Tight Junctions but Loose Associations?Digestive diseases and sciences · 2020Review
- Role for diet in normal gut barrier function: developing guidance within the framework of food-labeling regulations.American journal of physiology. Gastrointestinal and liver physiology · 2019Review
- Differential effects of Clostridium difficile toxins on ion secretion and cell integrity in human intestinal cells.Pediatric research · 2019Article
- The Intestinal Barrier in Parkinson's Disease: Current State of Knowledge.Journal of Parkinson's disease · 2019Review
- Network-Based Selection of Candidate Markers and Assays to Assess the Impact of Oral Immune Interventions on Gut Functions.Frontiers in immunology · 2019Article
- Neurons and Glia in the Enteric Nervous System and Epithelial Barrier Function.Physiology (Bethesda, Md.) · 2018Review
- Cell Biology of Tight Junction Barrier Regulation and Mucosal Disease.Cold Spring Harbor perspectives in biology · 2018Review
- Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids.Journal of visualized experiments : JoVE · 2017Article
- Human intestinal epithelium in a dish: Current models for research into gastrointestinal pathophysiology.United European gastroenterology journal · 2017Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
In the mid-20th century, Hans Ussing developed a chamber that allowed for the simultaneous measurement of current and labeled probe flux across epithelia. Using frog skin as a model, Ussing used his results to propose mechanisms of transcellular Na(+) and K(+) transport across apical (exterior/luminal) and basolateral (interior) membranes that is essentially unchanged today. Others took advantage of Ussing's chambers to study mucosal tissues, including bladder and intestines. It quickly became clear that, in some tissues, passive paracellular flux, i.e., across the tight junction, was an important component of overall transepithelial transport. Subsequent work demonstrated that activation of the apical Na(+)-glucose cotransporter SGLT1 regulated paracellular permeability such that intestinal paracellular transport could coordinate with and amplify transcellular transport. Intermediates in this process include activation of p38 MAPK, the apical Na(+)/H(+) exchanger NHE3, and myosin light chain kinase (MLCK). Investigators then focused on these processes in disease. They found that TNF induces barrier dysfunction via MLCK activation and downstream caveolin-1-dependent endocytosis of the tight junction protein occludin. TNF also inhibited NHE3, and both barrier loss and PKCα-dependent NHE3 inhibition were required for TNF-induced acute diarrhea, emphasizing the interplay between transcellular and paracellular transport. Finally, studies using immune-mediated inflammatory bowel disease models showed that mice lacking epithelial MLCK were initially protected, but became ill as epithelial damage progressed and provided a tight junction-independent means of barrier loss. None of these advances would have been possible without the insights provided by Ussing and others using Ussing's ingenious, and still useful, chambers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.