ArticleForensic science international. Genetics2016
Sequence variation of 22 autosomal STR loci detected by next generation sequencing.
Article in Forensic science international. Genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 167 citations in OpenAlex.
- High polymorphism detected by massively parallel sequencing of autosomal STRs using old blood samples from a Chinese Han population.Scientific reports · 2019Trial
- Assessing direct FTA cards for human identification using Oxford nanopore sequencing.International journal of legal medicine · 2026Article
- Development and validation of a STR and SNP multiplex detection system (88 STRs and 348 SNPs) using massively parallel sequencing.International journal of legal medicine · 2026Article
- Autosomal STR Markers for Forensic Genetics: Applications, Challenges, and Future Directions.Genes · 2026Review
- STRspy2.0: Unlocking the Potential of Long Reads for Forensic DNA Profiling.International journal of molecular sciences · 2026Article
- Development of a Multiplex Amplification System Using Oxford Nanopore Sequencing for STRs and InDels.Human mutation · 2026Article
- Evaluation of gain in statistical power for kinship analysis using sequence-based versus length-based STR genotyping.International journal of legal medicine · 2026Article
- Article
- Navigating triplet repeats sequencing: concepts, methodological challenges and perspective for Huntington's disease.Nucleic acids research · 2025Review
- Next-generation sequencing-based tools or nanopore-based tools: which is more suitable for short tandem repeats genotyping of nanopore sequencing?Bioinformatics advances · 2025Article
- Sequencing the orthologs of human autosomal forensic short tandem repeats provides individual- and species-level identification in African great apes.BMC ecology and evolution · 2024Article
- Characterization of sequence variations in the extended flanking regions using massively parallel sequencing in 21 A-STRs and 21 Y-STRs.BMC genomics · 2024Article
- Developmental validation of the STRSeqTyper122 kit for massively parallel sequencing of forensic STRs.International journal of legal medicine · 2024Article
- A novel 193-plex MPS panel integrating STRs and SNPs highlights the application value of forensic genetics in individual identification and paternity testing.Human genetics · 2024Article
- Forensic analysis and sequence variation of 133 STRs in the Hakka population.Frontiers in genetics · 2024Article
- Concordance study on Y-STRs typing between SeqStudio™ genetic analyzer for HID and MiSeq™ FGx forensic genomics system.Molecular biology reports · 2023Article
- Article
- Evaluation of Library Preparation Workflows and Applications to Different Sample Types Using the PowerSeqGenes · 2023Article
- Cost-Effective Next Generation Sequencing-Based STR Typing with Improved Analysis of Minor, Degraded and Inhibitor-Containing DNA Samples.International journal of molecular sciences · 2023Article
- Next generation sequencing of Y-STRs in father-son pairs and comparison with traditional capillary electrophoresis.Forensic sciences research · 2022Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Sequencing short tandem repeat (STR) loci allows for determination of repeat motif variations within the STR (or entire PCR amplicon) which cannot be ascertained by size-based PCR fragment analysis. Sanger sequencing has been used in research laboratories to further characterize STR loci, but is impractical for routine forensic use due to the laborious nature of the procedure in general and additional steps required to separate heterozygous alleles. Recent advances in library preparation methods enable high-throughput next generation sequencing (NGS) and technological improvements in sequencing chemistries now offer sufficient read lengths to encompass STR alleles. Herein, we present sequencing results from 183 DNA samples, including African American, Caucasian, and Hispanic individuals, at 22 autosomal forensic STR loci using an assay designed for NGS. The resulting dataset has been used to perform population genetic analyses of allelic diversity by length compared to sequence, and exemplifies which loci are likely to achieve the greatest gains in discrimination via sequencing. Within this data set, six loci demonstrate greater than double the number of alleles obtained by sequence compared to the number of alleles obtained by length: D12S391, D2S1338, D21S11, D8S1179, vWA, and D3S1358. As expected, repeat region sequences which had not previously been reported in forensic literature were identified.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.