ArticlePloS one2015
Dog Tear Film Proteome In-Depth Analysis.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Effect of tear collection on lacrimal total protein content in dogs and cats: a comparison between Schirmer strips and ophthalmic sponges.BMC veterinary research · 2018Trial
- Canine Tear Proteomics: A New Frontier in Veterinary Ophthalmology.Veterinary ophthalmology · 2026Review
- Proteomic Analysis of Tear Film in Dogs and Cats: Emerging Biomarkers of Cognitive Dysfunction and Neurodegenerative Disorders.Animals : an open access journal from MDPI · 2026Review
- A Pilot Proteomic Analysis of Tear Fluid in Domestic Cats with and Without Conjunctivitis Using MALDI-TOF/TOF Mass Spectrometry.Animals : an open access journal from MDPI · 2026Article
- Tear Protein Alteration in Dogs with Keratoconjunctivitis Sicca.Animals : an open access journal from MDPI · 2026Article
- Proteomic Analysis of Tear Films in Healthy Female and Male Dogs Using MALDI-TOF (Matrix Assisted Laser Desortion/Ionization Time-of-Flight) Mass Spectrometry.Animals : an open access journal from MDPI · 2025Article
- Analysis and comparison of tear protein profiles in dogs using different tear collection methods.BMC veterinary research · 2022Article
- Proteomic Analysis of Tear Film in Canine Diabetic Patients with And Without Retinopathy.Journal of veterinary research · 2022Article
- Mass spectrometry-based tear proteomics for noninvasive biomarker discovery.Mass spectrometry reviews · 2022Review
- Data-Independent Acquisition Enables Robust Quantification of 400 Proteins in Non-Depleted Canine Plasma.Proteomes · 2022Article
- Protein expression of the tear film of domestic cats before and after inoculation with Toxoplasma gondii.BMC veterinary research · 2021Article
- Changes in tear protein profile in dogs with keratoconjunctivitis sicca following topical treatment using cyclosporine A.Veterinary world · 2021Article
- Dexmedetomidine and Tear Production: Evaluation in Dogs as Spontaneous Model for Ocular Surface Disorders.Veterinary sciences · 2021Article
- Tear Film Proteome of Healthy Domestic Cats.Veterinary medicine international · 2021Article
- Proteomic Analysis of Tear Film Obtained from Diabetic Dogs.Animals : an open access journal from MDPI · 2020Article
- The proteomics of roadside hawk (Rupornis magnirostris), broad-snouted caiman (Caiman latirostris) and loggerhead sea turtle (Caretta caretta) tears.BMC veterinary research · 2020Article
- Comparative Analysis of Tear Composition in Humans, Domestic Mammals, Reptiles, and Birds.Frontiers in veterinary science · 2020Article
- Urinary proteome of dogs with kidney injury during babesiosis.BMC veterinary research · 2019Article
- Article
- Skin Mucus of Gilthead Sea Bream (Frontiers in physiology · 2017Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, mass spectrometry was used to explore the canine tear proteome. Tear samples were obtained from six healthy dogs, and one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (1D SDS-PAGE) was used as a first step to separate intact proteins into 17 bands. Each fraction was then trypsin digested and analysed by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF-MS/MS) to characterize the protein components in each fraction. In total, 125 tear proteins were identified, with MCA (Major Canine Allergen), Serum albumin, UPF0557 protein C10orf119 homolog, Collagen alpha-2(I) chain, Tyrosine -protein kinase Fer, Keratine type II cytoskeletal, Beta-crystallin B2, Interleukin-6 and Desmin occurring as the most confident ones with the highest scores. The results showed that the proteomic strategy used in this study was successful in the analysis of the dog tear proteome. To the best of our knowledge, this study is the first to report the comprehensive proteome profile of tears from healthy dogs by 1D SDS PAGE and MALDI-TOF. Data are available via ProteomeXchange with identifier PXD003124.
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