ArticleTissue engineering. Part A2016
Accelerated Chondrogenic Differentiation of Human Perivascular Stem Cells with NELL-1.
Article in Tissue engineering. Part A, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 27 citations in OpenAlex.
- Nell-1 regulates fibrocartilage stem cells and alleviates temporomandibular joint osteoarthritis progression by inhibiting hedgehog pathway.Journal of orthopaedic surgery and research · 2026Article
- NELL-1: role and mechanisms in disease pathogenesis and development.Frontiers in cell and developmental biology · 2026Review
- Modern experimental methods for assessing the effectiveness of tissue-engineered products for hyaline cartilage regeneration.Frontiers in bioengineering and biotechnology · 2025Review
- Role of NEL‑like molecule‑1 in osteogenesis/chondrogenesis (Review).International journal of molecular medicine · 2025Review
- Editorial: Chondrogenic potentials, protocols and mechanisms of mesenchymal progenitor cells.Frontiers in cell and developmental biology · 2023Article
- Runx2 and Nell-1 in dental follicle progenitor cells regulate bone remodeling and tooth eruption.Stem cell research & therapy · 2022Review
- Blood Vessel Resident Human Stem Cells in Health and Disease.Stem cells translational medicine · 2022Review
- The current state of the osteoarthritis drug development pipeline: a comprehensive narrative review of the present challenges and future opportunities.Therapeutic advances in musculoskeletal disease · 2022Review
- Updates on mesenchymal stem cell therapies for articular cartilage regeneration in large animal models.Frontiers in cell and developmental biology · 2022Review
- Males and Females Have Distinct Molecular Events in the Articular Cartilage during Knee Osteoarthritis.International journal of molecular sciences · 2021Article
- Research progress on tissue engineering in repairing tempomandibular joint.Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2021Article
- Assessing the genetic relationships between osteoarthritis and human plasma proteins: a large scale genetic correlation scan.Annals of translational medicine · 2020Article
- Cumulative inactivation of Nell-1 in Wnt1 expressing cell lineages results in craniofacial skeletal hypoplasia and postnatal hydrocephalus.Cell death and differentiation · 2020Article
- Neural EGFL like 1 as a potential pro-chondrogenic, anti-inflammatory dual-functional disease-modifying osteoarthritis drug.Biomaterials · 2020Article
- Nell-1 Is a Key Functional Modulator in Osteochondrogenesis and Beyond.Journal of dental research · 2019Review
- Perivascular Mesenchymal Progenitors for Bone Regeneration.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2019Review
- Robo2 contains a cryptic binding site for neural EGFL-like (NELL) protein 1/2.The Journal of biological chemistry · 2019Article
- Neural EGFL-Like 1 Regulates Cartilage Maturation through Runt-Related Transcription Factor 3-Mediated Indian Hedgehog Signaling.The American journal of pathology · 2018Article
- Microenvironmental factors that regulate mesenchymal stem cells: lessons learned from the study of heterotopic ossification.Histology and histopathology · 2017Review
- NELL-1 induces Sca-1+ mesenchymal progenitor cell expansion in models of bone maintenance and repair.JCI insight · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Osteoarthritis is the leading cause of disability in the US. Consequently, there is a pressing need for restoring the structural and functional properties of diseased articular cartilage. Yet the search for the right combination of proper target cells and growth factors for cartilage regeneration remains challenging. In this study, we first tested the intrinsic chondrogenic differentiation ability of human perivascular stem cells (hPSCs), a novel source of mesenchymal stem cells (MSCs) isolated by fluorescence-activated cell sorting (FACS) from human adipose tissue. A putative prochondrogenic growth factor, NEL-like molecule-1 (NELL-1), was added to the hPSC pellets to upregulate gene expression of chondrogenic markers, including AGGRECAN, COLLAGEN II, and COMP. Furthermore, the addition of NELL-1 to a transforming growth factor beta 3 (TGF-β3) + bone morphogenetic protein-6 (BMP-6) "cocktail" resulted in the best combinatorial stimulation in accelerating the chondrogenic differentiation of hPSCs, as evidenced by increased gene and protein expression of chondrogenic markers in a shortened induction time without elevating expression of hypertrophic, fibrotic, and osteogenic markers. Mechanistically, this acceleration rendered by NELL-1 may be partially attributed to NELL-1's upregulation of BMP receptors and TGF-β receptor type I in hPSCs for increased responsiveness to BMPs + TGF-βs. In conclusion, lipoaspirate-derived hPSCs present a novel and abundant cell source of MSCs for cartilage regeneration, and the combinatorial application of NELL-1, TGF-β3, and BMP-6 with hPSCs may remarkably enhance and accelerate cartilage repair.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.