ArticlePloS one2015
Vitamin E Analogue Improves Rabbit Sperm Quality during the Process of Cryopreservation through Its Antioxidative Action.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The trial behind it
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Who cites it
29 citing papers in PubMed, 67 citations in OpenAlex.
- L-Histidine Improves Boar Sperm Quality by Alleviating Oxidative Stress During Preservation at 17 °C.Antioxidants (Basel, Switzerland) · 2026Article
- Reactive oxygen species and antioxidant strategies in human sperm cryodamage.Frontiers in medicine · 2026Review
- Silymarin as a promising antioxidant for sperm cryopreservation: a narrative review.Translational andrology and urology · 2025Review
- Cryoprotectant effects of mangiferin on the quality of frozen-thawed boar sperm.Frontiers in veterinary science · 2025Article
- Repeated injections of vitamin E and Se improves testicular morphology, testosterone and in vitro and in vivo sperm fertility in subfertile rabbits.Veterinary research communications · 2024Article
- The effects of vitamin E supplementation on sperm parameters, chromatin integrity, and gene expression before and after freezing in aged mice.Clinical and experimental reproductive medicine · 2024Article
- Sperm freezing damage: the role of regulated cell death.Cell death discovery · 2024Review
- Pyrroloquinoline Quinone Improves Ram Sperm Quality through Its Antioxidative Ability during Storage at 4 °C.Antioxidants (Basel, Switzerland) · 2024Article
- Effects of sodium selenite, cysteamine, bacterially synthesized Se-NPs, and cysteamine loaded on Se-NPs on ram sperm cryopreservation.Scientific reports · 2024Article
- Effect of sulforaphane on long-term storage of rabbit semen.Animal reproduction · 2023Article
- Effects of riboflavin on boar sperm motility, sperm quality, enzyme activity and antioxidant status during cryopreservation.Veterinary medicine and science · 2022Article
- Pre-treatment of ram semen extender with magnetic nanoparticles on freeze-thawed spermatozoa.Veterinary medicine and science · 2022Article
- Cysteine improves boar sperm quality via glutathione biosynthesis during the liquid storage.Animal bioscience · 2022Article
- Resveratrol and lycium barbarum polysaccharide improve Qinling giant panda (Ailuropoda melanoleuca Qinlingensis) sperm quality during cryopreservation.BMC veterinary research · 2022Article
- Reducing the Glucose Level in Pre-treatment Solution Improves Post-thaw Boar Sperm Quality.Frontiers in veterinary science · 2022Article
- Metabolomic analysis reveals spermatozoa and seminal plasma differences between Duroc and Liang guang Small-spotted pig.Frontiers in veterinary science · 2022Article
- Improving the post-thaw quality of rooster semen using the extender supplemented with resveratrol.Poultry science · 2021Article
- Protective Effect of Chlorogenic Acid on Human Sperm: In Vitro Studies and Frozen-Thawed Protocol.Antioxidants (Basel, Switzerland) · 2021Article
- Strategies for Highly Efficient Rabbit Sperm Cryopreservation.Animals : an open access journal from MDPI · 2021Review
- Mitochondrial Dysfunction and Oxidative Stress Caused by Cryopreservation in Reproductive Cells.Antioxidants (Basel, Switzerland) · 2021Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The process of cryopreservation results in high concentration of reactive oxygen species which is detrimental to spermatozoa. The aim of this study was to investigate whether addition of vitamin E analogue to freezing extender can facilitate the cryosurvival of spermatozoa in rabbits, and how vitamin E protects spermatozoa against damages during the process of preservation. Freshly ejaculated semen was diluted with Tris-citrate-glucose extender supplemented with different concentrations of Trolox (a vitamin E analogue). The level of radical oxygen species (ROS) in spermatozoa that was exposed to Trolox was significantly lower than that of the control during each step of the process of preservation. The percentage of frozen-thawed spermatozoa with lipid peroxidation in the Trolox treatments was significantly lower than that of the control. The motility, intact acrosome, membrane integrity and mitochondrial potentials of the frozen-thawed spermatozoa in the treatment of 200 μM Trolox were significantly higher than those of the control. These observations suggest that addition of vitamin E to a freezing extender leads to higher integrity of acrosome, plasma membrane and mitochondrial membrane potential as well as higher motility. Vitamin E protects spermatozoa through its capacity to quench ROS accumulation and lipid peroxidation during the process of preservation. Addition of Trolox is recommended to facilitate the improvement of semen preservation for the rabbit breeding industry.
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