ArticleJournal of orthopaedic research : official publication of the Orthopaedic Research Society2016
Contrast-enhanced CT using a cationic contrast agent enables non-destructive assessment of the biochemical and biomechanical properties of mouse tibial plateau cartilage.
Article in Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 56 citations in OpenAlex.
- An immunoregenerative approach to mitigate post-traumatic osteoarthritis after intra-articular fracture.Bone & joint research · 2026Article
- Assessment of morphology, radiopacity gradient and mechanical properties of articular cartilage with contrast-enhanced high-resolution peripheral quantitative computed tomography.Frontiers in bioengineering and biotechnology · 2026Article
- Assessment of CA4+ Impact on Mechanical Properties of Articular Cartilage.Materials (Basel, Switzerland) · 2025Article
- Photon-counting in dual-contrast-enhanced computed tomography: a proof-of-concept quantitative biomechanical assessment of articular cartilage.Scientific reports · 2024Article
- Iodinated gadolinium-gold nanomaterial as a multimodal contrast agent for cartilage tissue imaging.APL bioengineering · 2024Article
- Computed Tomography of Cartilage: An Exploration of Novel Cationic Bismuth Contrast Agent.Annals of biomedical engineering · 2023Article
- Evaluation of a lanthanide nanoparticle-based contrast agent for microcomputed tomography of porous channels in subchondral bone.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2023Article
- Contrast-enhanced micro-computed tomography of compartment and time-dependent changes in femoral cartilage and subchondral plate in a murine model of osteoarthritis.Anatomical record (Hoboken, N.J. : 2007) · 2023Article
- A Cationic Contrast Agent in X-ray Imaging of Articular Cartilage: Pre-Clinical Evaluation of Diffusion and Attenuation Properties.Diagnostics (Basel, Switzerland) · 2022Article
- Contrast solution properties and scan parameters influence the apparent diffusivity of computed tomography contrast agents in articular cartilage.Journal of the Royal Society, Interface · 2022Article
- Contrast-Enhanced Micro-Computed Tomography for 3D Visualization and Quantification of Glycosaminoglycans in Different Cartilage Types.Cartilage · 2021Article
- Quantitative Evaluation of Equine Articular Cartilage Using Cationic Contrast-Enhanced Computed Tomography.Cartilage · 2021Article
- Effects of human articular cartilage constituents on simultaneous diffusion of cationic and nonionic contrast agents.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2021Article
- Accelerating functional gene discovery in osteoarthritis.Nature communications · 2021Article
- Influence of fixation on CA4+ contrast enhanced microCT of articular cartilage and subsequent feasibility for histological evaluation.American journal of translational research · 2021Article
- Challenges and opportunities for small volumes delivery into the skin.Biomicrofluidics · 2021Article
- Contrast-enhanced micro-computed tomography of articular cartilage morphology with ioversol and iomeprol.Journal of anatomy · 2020Article
- dGEMRIC and CECT Comparison of Cationic and Anionic Contrast Agents in Cadaveric Human Metacarpal Cartilage.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2020Article
- Non-Destructive Spectroscopic Assessment of High and Low Weight Bearing Articular Cartilage Correlates with Mechanical Properties.Cartilage · 2019Article
- In Vivo Tracking of Tissue Engineered Constructs.Micromachines · 2019Review
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Mouse models of osteoarthritis (OA) are commonly used to study the disease's pathogenesis and efficacy of potential treatments. However, measuring the biochemical and mechanical properties of articular cartilage in these models currently requires destructive and time-consuming histology and mechanical testing. Therefore, we examined the feasibility of using contrast-enhanced CT (CECT) to rapidly and non-destructively image and assess the glycosaminoglycan (GAG) content. Using three ex vivo C57BL/6 mouse tibial plateaus, we determined the time required for the cationic contrast agent CA4+ to equilibrate in the cartilage. The whole-joint coefficient of friction (μ) of 10 mouse knees (some digested with Chondroitenase ABC to introduce variation in GAG) was evaluated using a modified Stanton pendulum. For both the medial and lateral tibial plateau cartilage of these knees, linear regression was used to compare the equilibrium CECT attenuations to μ, as well as each side's indentation equilibrium modulus (E) and Safranin-O determined GAG content. CA4+ equilibrated in the cartilage in 30.9 ± 0.95 min (mean ± SD, tau value of 6.17 ± 0.19 min). The mean medial and lateral CECT attenuation was correlated with μ (R(2) = 0.69, p < 0.05), and the individual medial and lateral CECT attenuations correlated with their respective GAG contents (R(2) ≥ 0.63, p < 0.05) and E (R(2) ≥ 0.63, p < 0.05). In conclusion, CECT using CA4+ is a simple, non-destructive technique for three-dimensional imaging of ex vivo mouse cartilage, and significant correlations between CECT attenuation and GAG, E, and μ are observed. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:1130-1138, 2016.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.