ArticleSpringerPlus2015
Characterization of mannanase from Bacillus circulans NT 6.7 and its application in mannooligosaccharides preparation as prebiotic.
Article in SpringerPlus, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 67 citations in OpenAlex.
- A Novel GH26 β-Mannanase from Bacillus subtilis HM7 Producing Manno-Oligosaccharides with Strain-Specific Prebiotic Activity.Applied biochemistry and biotechnology · 2026Article
- Gut Microbiota Modulation in CNS Disorders: Therapeutic Strategies and Implications for Drug Efficacy.Molecular neurobiology · 2026Review
- Advances in molecular mechanisms and therapeutic strategies for central nervous system diseases based on gut microbiota imbalance.Journal of advanced research · 2025Review
- Discovery and Characterization of Mannan-Specialized GH5 Endo-1,4-β-mannanases: a Strategy for Açaí (Journal of agricultural and food chemistry · 2025Article
- Evaluation of Acute and Subchronic Oral Toxicity of Copra Meal Hydrolysate: A Novel Candidate for Prebiotic in Sprague Dawley Rats.Journal of toxicology · 2025Article
- Manno-oligosaccharides as a promising antimicrobial strategy: pathogen inhibition and synergistic effects with antibiotics.Frontiers in microbiology · 2025Article
- Research and application progress of microbial β-mannanases: a mini-review.World journal of microbiology & biotechnology · 2024Review
- Recent Advances in Biomedical Applications of Mannans and Xylans.Current drug targets · 2024Review
- Article
- Dietary Mannanoligosaccharide Supplementation Improves Growth Performance, Intestinal Integrity, Serum Immunity, and Antioxidant Capacity of Partridge Shank Chickens.The journal of poultry science · 2021Article
- Article
- Nutritional improvement of copra meal using mannanase and3 Biotech · 2020Article
- Copra meal hydrolysis by the recombinant β-mannanase KMAN-3 and MAN 6.7 expressed in3 Biotech · 2020Article
- Article
- Article
- Structural basis of exo-β-mannanase activity in the GH2 family.The Journal of biological chemistry · 2018Article
- Optimization of hydrolysis conditions for the mannooligosaccharides copra meal hydrolysate production.3 Biotech · 2018Article
- Article
- Purification, characterization, and overexpression of an endo-1,4-β-mannanase from thermotolerant Bacillus sp. SWU60.World journal of microbiology & biotechnology · 2017Article
- Cloning, secretory expression and characterization of recombinant β-mannanase from Bacillus circulans NT 6.7.SpringerPlus · 2014Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study focused on the characterization of mannanase from Bacillus circulans NT 6.7 for mannooligosaccharides (MOS) production. The enzyme from B. circulans NT 6.7 was produced using defatted copra meal as a carbon source. The mannanase was purified by ultrafiltration and column chromatography of Q-Sepharose. The purified protein (M1) was a dimeric protein with a 40 kDa subunit. The purified M1 exhibited optimum pH and temperature at pH 6.0 and 60 °C, respectively. It was activated by Mn(2+,) Mg(2+,) and Cu(2+), and as inhibited by EDTA (45-65 %). The purified enzyme exhibited high specificity to beta-mannan: konjac (glucomannan), locust bean gum (galactomannan), ivory nut (mannan), guar gum (galactomannan) and defatted copra meal (galactomannan). The defatted copra meal could be hydrolyzed by purified M1 into mannooligosaccharides which promoted beneficial bacteria, especially Lactobacillus group, and inhibited pathogenic bacteria; Shigella dysenteria DMST 1511, Staphylococcus aureus TISTR 029, and Salmonella enterica serovar Enteritidis DMST 17368. Therefore, the mannanase from B. circulans NT 6.7 would be a novel source of enzymes for the mannooligosaccharides production as prebiotics.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.