Evidence map›Paper›PMID 26697278›Full record

ArticleApplications in plant sciences2015

Development of microsatellite markers for the endangered Pedicularis ishidoyana (Orobanchaceae) using next-generation sequencing.

Won-Bum Cho, In-Su Choi, Byoung-Hee Choi

Abstract read
In one paragraph

Article in Applications in plant sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Plants (Basel, Switzerland) · 2019
    Review
  3. Development of 26 microsatellite markers inApplications in plant sciences · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Won-Bum ChoDepartment of Biological Sciences, Inha University, Incheon 402-751, Republic of Korea.
In-Su ChoiDepartment of Biological Sciences, Inha University, Incheon 402-751, Republic of Korea.
Byoung-Hee ChoiDepartment of Biological Sciences, Inha University, Incheon 402-751, Republic of Korea.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

premise of the studyMicrosatellite primers were developed for Pedicularis ishidoyana (Orobanchaceae), an endangered and hemiparasitic plant that is narrowly endemic to Korea. Because its populations are threatened by loss of suitable habitat, conservation efforts are required. METHODS AND

resultsWe developed polymorphic microsatellite loci through reference mapping of 300-bp paired-end reads obtained from Illumina MiSeq data. In all, 74 primer pairs were designed and 32 were amplified. Of these, 18 pairs were polymorphic, with two to six alleles each occurring in 26 individual plants. Expected and observed heterozygosities ranged from 0.142 to 0.703 and from 0.077 to 0.615, respectively.

conclusionsThese microsatellite markers are expected to be useful for studies of the population genetics of P. ishidoyana.

Indexed as

endangered speciesmicrosatellitenext-generation sequencing (NGS)OrobanchaceaePedicularis ishidoyana

Identifiers

PMID26697278
PMCPMC4683043

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.