Evidence map›Paper›PMID 26697157›Full record

ArticleIranian journal of microbiology2015

Distribution of Shiga toxin genes subtypes in B1 phylotypes of Escherichia coli isolated from calves suffering from diarrhea in Tehran suburb using DNA oligonucleotide arrays.

Hamid Staji, Alfreda Tonelli, Abbas Javaheri-Vayeghan, Emad Changizi, Mohammad Reza Salimi-Bejestani

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Article in Iranian journal of microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.4field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

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3 · Its place in the literature

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1 citing paper in PubMed, 8 citations in OpenAlex.

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4 · The record

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5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Hamid StajiDepartment of Pathobiology, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran.
Alfreda TonelliIstituto Zooprofilattico Sperimentaledell'Abruzzo e del Molise " G. Caporale", Research and Development, Campo Boario, 64100 Teramo, Italy.
Abbas Javaheri-VayeghanDepartment of Pathobiology, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran.
Emad ChangiziDepartment of Pathobiology, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran.
Mohammad Reza Salimi-BejestaniDepartment of Pathobiology, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran.
Semnan University · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND AND

objectivesShiga toxin-producing Escherichia coli (STEC) have emerged as human pathogens and contamination via animal origin has been a major public health concern. We compared the distribution of phylogenetic groups and prevalence of stx gene variants among the pathogenic strains of Escherichia coli isolated from feces of diarrheatic calves in Tehran suburb farms. MATERIALS AND

methodsIn this study we screened 140 diarrheatic calves (1-15 days old) for E. coli strains during a 3 months period of time. The isolated strains were grouped into different phylotypes according to the presence of chuA, yjaA and TSPE4.C2 genes. Then, the prevalence of stx gene subtypes was evaluated in the B1 phylotypes.

resultsFrom diarrheatic calves, 51 bacterial isolates were biochemically identified as E. coli and 31 isolates out of 51 were considered B1 phylotype using DNA Microarray technology. Of these isolates, 20 contained stx1a and stx1b and one harbored all mentioned variants of stx genes except stx2b2 .

conclusionThis study showed that in Tehran suburb, the B1 phylotype of E. coli is prevalent as a causative agent of diarrhea in calves and the prevalence of stx1 gene subtypes is dominant in comparison with other subtypes. Considering the possibility that these stx genes can be spread to other strains, bovine E. coli strains are an important source of stx genes for other strains and further study and surveillance seems to be required for the exact identification of virulence profile of E. coli phylotypes in different hosts.

Indexed as

B1 phylotypecalf diarrheaEscherichia colishiga-like toxin subtypesTehran suburb

Identifiers

PMID26697157
PMCPMC4685162
OpenAlexW2104258378

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.