ArticleIranian journal of microbiology2015
Development of a loop-mediated isothermal amplification assay for rapid detection of Yersinia enterocolitica via targeting a conserved locus.
Article in Iranian journal of microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Immunogenicity and safety of subunit plague vaccine: A randomized phase 2a clinical trial.Human vaccines & immunotherapeutics · 2016Trial
- Development of a cost-effective LAMP method for screening and monitoring enteropathogenicScience in One Health · 2025Article
- Development and Validation of the MAST ISOPLEXInternational journal of molecular sciences · 2024Article
- Comparative assessment of two in-house-built isothermal assays for visual detection of African swine fever virus.Journal of biosciences · 2024Article
- Establishment of a Real-Time Recombinase Polymerase Amplification for Rapid Detection of PathogenicPathogens (Basel, Switzerland) · 2023Article
- Comparative analysis of loop-mediated isothermal amplification combined with microfluidic chip technology and q-PCR in the detection of clinical infectious pathogens.Journal of clinical laboratory analysis · 2022Article
- A Loop-Mediated Isothermal Amplification (LAMP) Assay Specific toMicroorganisms · 2022Article
- The Sensitivity and Specificity of Loop-Mediated Isothermal Amplification and PCR Methods in Detection of Foodborne Microorganisms: A Systematic Review and Meta-Analysis.Iranian journal of public health · 2021Review
- Development of a LAMP assay using a portable device for the real-time detection of cotton leaf curl disease in field conditions.Biology methods & protocols · 2021Article
- Development and evaluation of loop-mediated isothermal amplification for detection of Yersinia pestis in plague biological samples.PloS one · 2020Article
- Article
- One year immunogenicity and safety of subunit plague vaccine in Chinese healthy adults: An extended open-label study.Human vaccines & immunotherapeutics · 2018Article
- Yersinia pestis detection by loop-mediated isothermal amplification combined with magnetic bead capture of DNA.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
objectivesLoop-mediated isothermal amplification is a novel nucleic acid amplification assay providing as a simple diagnostic tool for rapid identification of microbial diseases in developing countries. In this study, a LAMP assay was established for Yersinia enterocolitica, a leading cause of acute enterocolitis in young children. MATERIALS AND
methodsLAMP assay was established with four primers targeting a specific locus for the detection of Y. enterocolitica. The assay was conducted at 65°C in thermo block for 90min. The sensitivity of LAMP was evaluated in comparison to conventional PCR using pTZ57R containing the target locus. Finally, specificity was assessed using DNA from common enteropathogenic bacteria.
resultsResults showed that the sensitivity of LAMP assay was 44-copy number, which was 10-fold higher than that of PCR. No cross-reactivity was observed when testing against other enteropathogenic pathogens.
conclusionThis study showed that LAMP assay is an alternative molecular diagnostic tool for infections with Y. enterocolitica. In addition, this method may be useful in diagnosis at field or in laboratories without PCR machine.
Indexed as
Identifiers
26697156PMC4685161W2104627129What OpenQuestion holds
Registered trials
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