ArticleFrontiers in plant science2015
Isolation of Plant Photosystem II Complexes by Fractional Solubilization.
Article in Frontiers in plant science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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Who cites it
10 citing papers in PubMed, 18 citations in OpenAlex.
- How state transitions balance photosynthetic electron transport in plants - a quantitative study.The New phytologist · 2026Article
- A step closer to fully understand how the engine of life is repaired from damages caused by its fuel.Plant physiology · 2023Article
- Bicarbonate activation of the monomeric photosystem II-PsbS/Psb27 complex.Plant physiology · 2023Article
- Investigation of Photosystem II Functional Size in Higher Plants under Physiological and Stress Conditions Using Radiation Target Analysis and Sucrose Gradient Ultracentrifugation.Molecules (Basel, Switzerland) · 2022Article
- Solubilization Method for Isolation of Photosynthetic Mega- and Super-complexes from Conifer Thylakoids.Bio-protocol · 2021Article
- Light-driven formation of manganese oxide by today's photosystem II supports evolutionarily ancient manganese-oxidizing photosynthesis.Nature communications · 2020Article
- Specific Lhc Proteins Are Bound to PSI or PSII Supercomplexes in the DiatomPlant physiology · 2020Article
- Elevated COBMC plant biology · 2019Article
- RhVI1 is a membrane-anchored vacuolar invertase highly expressed in Rosa hybrida L. petals.Journal of experimental botany · 2016Article
- The Use of Advanced Mass Spectrometry to Dissect the Life-Cycle of Photosystem II.Frontiers in plant science · 2016Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Photosystem II (PSII) occurs in different forms and supercomplexes in thylakoid membranes. Using a transplastomic strain of Nicotiana tabacum histidine tagged on the subunit PsbE, we have previously shown that a mild extraction protocol with β-dodecylmaltoside enriches PSII characteristic of lamellae and grana margins. Here, we characterize residual granal PSII that is not extracted by this first solubilization step. Using affinity purification, we demonstrate that this PSII fraction consists of PSII-LHCII mega- and supercomplexes, PSII dimers, and PSII monomers, which were separated by gel filtration and functionally characterized. Our findings represent an alternative demonstration of different PSII populations in thylakoid membranes, and they make it possible to prepare PSII-LHCII supercomplexes in high yield.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.