ArticleFrontiers in plant science2015
Proteomic Analysis of a Fraction with Intact Eyespots of Chlamydomonas reinhardtii and Assignment of Protein Methylation.
Article in Frontiers in plant science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Proteomic characterization of a lutein-hyperaccumulating Chlamydomonas reinhardtii mutant reveals photoprotection-related factors as targets for increasing cellular carotenoid content.Biotechnology for biofuels and bioproducts · 2023Article
- Chlamydomonas reinhardtii cellular compartments and their contribution to intracellular calcium signalling.Journal of experimental botany · 2021Review
- Plastoglobular protein 18 is involved in chloroplast function and thylakoid formation.Journal of experimental botany · 2019Article
- A Series of Fortunate Events: Introducing Chlamydomonas as a Reference Organism.The Plant cell · 2019Review
- Channelrhodopsin-1 Phosphorylation Changes with Phototactic Behavior and Responds to Physiological Stimuli inThe Plant cell · 2019Article
- A Musashi Splice Variant and Its Interaction Partners Influence Temperature Acclimation inPlant physiology · 2018Article
- Heuristic Modeling and 3D Stereoscopic Visualization of a Chlamydomonas reinhardtii Cell.Journal of integrative bioinformatics · 2018Article
- Article
- Protein arginine methyltransferases interact with intraflagellar transport particles and change location during flagellar growth and resorption.Molecular biology of the cell · 2017Article
- Metabolic responses to ethanol and butanol inBiotechnology for biofuels · 2017Article
- Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Flagellate green algae possess a visual system, the eyespot. In Chlamydomonas reinhardtii it is situated at the edge of the chloroplast and consists of two carotenoid rich lipid globule layers subtended by thylakoid membranes (TM) that are attached to both chloroplast envelope membranes and a specialized area of the plasma membrane (PM). A former analysis of an eyespot fraction identified 203 proteins. To increase the understanding of eyespot related processes, knowledge of the protein composition of the membranes in its close vicinity is desirable. Here, we present a purification procedure that allows isolation of intact eyespots. This gain in intactness goes, however, hand in hand with an increase of contaminants from other organelles. Proteomic analysis identified 742 proteins. Novel candidates include proteins for eyespot development, retina-related proteins, ion pumps, and membrane-associated proteins, calcium sensing proteins as well as kinases, phosphatases and 14-3-3 proteins. Methylation of proteins at Arg or Lys is known as an important posttranslational modification involved in, e.g., signal transduction. Here, we identify several proteins from eyespot fractions that are methylated at Arg and/or Lys. Among them is the eyespot specific SOUL3 protein that influences the size and position of the eyespot and EYE2, a protein important for its development.
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