ArticleFrontiers in plant science2015
Polyamine and Its Metabolite H2O2 Play a Key Role in the Conversion of Embryogenic Callus into Somatic Embryos in Upland Cotton (Gossypium hirsutum L.).
Article in Frontiers in plant science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 52 citations in OpenAlex.
- Polyamine-Related Gene Families Identification and Regulatory Effects on Early Somatic Embryogenesis via Modulating Gene Expressions and Hormone Levels inPlants (Basel, Switzerland) · 2026Article
- Optimization of Somatic Embryogenesis and Transcriptomic Analysis of the Early Stage of Callus Redifferentiation inPlants (Basel, Switzerland) · 2025Article
- Spermine Promotes the Formation of Conchosporangia inMarine drugs · 2025Article
- CuCells · 2025Article
- 5-AzaCytidine Promotes Somatic Embryogenesis ofPlants (Basel, Switzerland) · 2025Article
- Spatiotemporal transcriptome and metabolome landscapes of cotton somatic embryos.Nature communications · 2025Article
- Polyamines: pleiotropic molecules regulating plant development and enhancing crop yield and quality.Plant biotechnology journal · 2024Review
- Physiological, Metabolic, and Transcriptomic Analyses Reveal Mechanisms of Proliferation and Somatic Embryogenesis of Litchi (International journal of molecular sciences · 2024Article
- HInternational journal of molecular sciences · 2024Article
- Comparative transcriptome analysis of high- and low-embryogenic Hevea brasiliensis genotypes reveals involvement of phytohormones in somatic embryogenesis.BMC plant biology · 2023Article
- Apomixis: oh, what a tangled web we have!Planta · 2023Review
- Polyamine Oxidase-Generated Reactive Oxygen Species in Plant Development and Adaptation: The Polyamine Oxidase-NADPH Oxidase Nexus.Antioxidants (Basel, Switzerland) · 2022Review
- Article
- Genome-Wide Identification and Functional Analysis of Polyamine Oxidase Genes in Maize Reveal Essential Roles in Abiotic Stress Tolerance.Frontiers in plant science · 2022Article
- Polyamine Metabolism Is Involved in the Direct Regeneration of Shoots from Arabidopsis Lateral Root Primordia.Plants (Basel, Switzerland) · 2021Article
- Somatic Embryo Yield and Quality From Norway Spruce Embryogenic Tissue Proliferated in Suspension Culture.Frontiers in plant science · 2021Article
- miRNAs as key regulators via targeting the phytohormone signaling pathways during somatic embryogenesis of plants.3 Biotech · 2020Review
- Ectopic expression of GhSAMDCScientific reports · 2020Article
- Different Roles of Auxins in Somatic Embryogenesis Efficiency in TwoInternational journal of molecular sciences · 2020Article
- NAA at a high concentration promotes efficient plant regeneration via direct somatic embryogenesis and SE-mediated transformation system in Ranunculus sceleratus.Scientific reports · 2019Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The objective of this study was to increase understanding about the mechanism by which polyamines (PAs) promote the conversion of embryogenic calli (EC) into somatic embryos in cotton (Gossypium hirsutum L.). We measured the levels of endogenous PAs and H2O2, quantified the expression levels of genes involved in the PAs pathway at various stages of cotton somatic embryogenesis (SE), and investigated the effects of exogenous PAs and H2O2 on differentiation and development of EC. Putrescine (Put), spermidine (Spd), and spermine (Spm) significantly increased from the EC stage to the early phase of embryo differentiation. The levels of Put then decreased until the somatic embryo stage whereas Spd and Spm remained nearly the same. The expression profiles of GhADC genes were consistent with changes in Put during cotton SE. The H2O2 concentrations began to increase significantly at the EC stage, during which time both GhPAO1 and GhPAO4 expressions were highest and PAO activity was significantly increased. Exogenous Put, Spd, Spm, and H2O2 not only enhanced embryogenic callus growth and embryo formation, but also alleviated the effects of D-arginine and 1, 8-diamino-octane, which are inhibitors of PA synthesis and PAO activity. Overall, the results suggest that both PAs and their metabolic product H2O2 are essential for the conversion of EC into somatic embryos in cotton.
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