ArticleFrontiers in microbiology2015
Rapid Identification of Emerging Human-Pathogenic Sporothrix Species with Rolling Circle Amplification.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Sporotrichosis in Mexico.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Pooled it
- Biogenic Silver Nanoparticle: In vitro antifungal activity against Sporothrix brasiliensis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Extracutaneous sporotrichosis.Clinical microbiology reviews · 2025Review
- Diagnosis of Human Endemic Mycoses Caused by Thermally Dimorphic Fungi: From Classical to Molecular Methods.Journal of fungi (Basel, Switzerland) · 2024Review
- Development and evaluation of a rapid diagnostic method for Sporothrix globosa in Asia using quantitative real-time PCR.BMC infectious diseases · 2024Article
- Clinical Analysis of Patients Diagnosed with Cutaneous Sporotrichosis in China.Infection and drug resistance · 2024Article
- Article
- Development of a Multiplex qPCR Assay for Fast Detection and Differentiation of Paracoccidioidomycosis Agents.Journal of fungi (Basel, Switzerland) · 2023Article
- A Spotlight on Sporothrix and Sporotrichosis.Mycopathologia · 2022Article
- Trends in Molecular Diagnostics and Genotyping Tools Applied for EmergingJournal of fungi (Basel, Switzerland) · 2022Review
- Current Progress on Epidemiology, Diagnosis, and Treatment of Sporotrichosis and Their Future Trends.Journal of fungi (Basel, Switzerland) · 2022Review
- A neglected disease. Human sporotrichosis in a densely populated urban area in São Paulo, Brazil: clinical-epidemiological and therapeutic aspects.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Article
- Molecular Diagnosis of Two Major Implantation Mycoses: Chromoblastomycosis and Sporotrichosis.Journal of fungi (Basel, Switzerland) · 2022Review
- Biological and Clinical Attributes ofInfection and drug resistance · 2022Review
- Article
- Guideline for the management of feline sporotrichosis caused by Sporothrix brasiliensis and literature revision.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Review
- A New Duplex PCR-Assay for the Detection and Identification ofJournal of fungi (Basel, Switzerland) · 2021Article
- Environmental Screening ofJournal of fungi (Basel, Switzerland) · 2020Article
- A One Health Approach to CombattingJournal of fungi (Basel, Switzerland) · 2020Review
- The threat of emerging and re-emerging pathogenic Sporothrix species.Mycopathologia · 2020Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sporothrix infections are emerging as an important human and animal threat among otherwise healthy patients, especially in Brazil and China. Correct identification of sporotrichosis agents is beneficial for epidemiological surveillance, enabling implementation of adequate public-health policies and guiding antifungal therapy. In areas of limited resources where sporotrichosis is endemic, high-throughput detection methods that are specific and sensitive are preferred over phenotypic methods that usually result in misidentification of closely related Sporothrix species. We sought to establish rolling circle amplification (RCA) as a low-cost screening tool for species-specific identification of human-pathogenic Sporothrix. We developed six species-specific padlock probes targeting polymorphisms in the gene encoding calmodulin. BLAST-searches revealed candidate probes that were conserved intraspecifically; no significant homology with sequences from humans, mice, plants or microorganisms outside members of Sporothrix were found. The accuracy of our RCA-based assay was demonstrated through the specificity of probe-template binding to 25 S. brasiliensis, 58 S. schenckii, 5 S. globosa, 1 S. luriei, 4 S. mexicana, and 3 S. pallida samples. No cross reactivity between closely related species was evident in vitro, and padlock probes yielded 100% specificity and sensitivity down to 3 × 10(6) copies of the target sequence. RCA-based speciation matched identifications via phylogenetic analysis of the gene encoding calmodulin and the rDNA operon (kappa 1.0; 95% confidence interval 1.0-1.0), supporting its use as a reliable alternative to DNA sequencing. This method is a powerful tool for rapid identification and specific detection of medically relevant Sporothrix, and due to its robustness has potential for ecological studies.
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