ArticlePLoS biology2015
Plants Encode a General siRNA Suppressor That Is Induced and Suppressed by Viruses.
Article in PLoS biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 51 citations in OpenAlex.
- Presence and function of small RNAs during plant reproduction.RNA biology · 2026Review
- Cell-type-specific ablation of siRNAs by Arabidopsis RTL1 reveals a role of phloem companion cells in systemic post-transcriptional gene silencing.Nucleic acids research · 2026Article
- Advances in Plant Antiviral RNAi: From Host DCLs/RDRs to Diversified Viral Counteracting Strategies.Viruses · 2026Review
- Atypical epigenetic and small RNA control of degenerated transposons and their fragments in clonally reproducingGenome research · 2025Article
- RH3 enhances antiviral defense by facilitating small RNA loading into Argonaute 2 at endoplasmic reticulum-chloroplast membrane contact sites.Nature communications · 2025Article
- Pseudouridine guides germline small RNA transport and epigenetic inheritance.Nature structural & molecular biology · 2025Article
- RNA interference and turnover in plants -a complex partnership.Frontiers in plant science · 2025Review
- Targeted suppression of siRNA biogenesis in Arabidopsis pollen promotes triploid seed viability.Nature communications · 2024Article
- Rhizospheric miRNAs affect the plant microbiota.ISME communications · 2024Article
- Toward Transgene-Free Transposon-Mediated Biological Mutagenesis for Plant Breeding.International journal of molecular sciences · 2023Review
- TYMV and TRV infect Arabidopsis thaliana by expressing weak suppressors of RNA silencing and inducing host RNASE THREE LIKE1.PLoS pathogens · 2023Article
- Roles of RNA silencing in viral and non-viral plant immunity and in the crosstalk between disease resistance systems.Nature reviews. Molecular cell biology · 2022Review
- RNase III, Ribosome Biogenesis and Beyond.Microorganisms · 2021Review
- Characterization of a DCL2-InsensitiveViruses · 2020Article
- Redox Components: Key Regulators of Epigenetic Modifications in Plants.International journal of molecular sciences · 2020Review
- RNA silencing ofBiotechnology reports (Amsterdam, Netherlands) · 2019Article
- NS3 Protein from Rice stripe virus affects the expression of endogenous genes in Nicotiana benthamiana.Virology journal · 2018Article
- Plant Responses to Pathogen Attack: Small RNAs in Focus.International journal of molecular sciences · 2018Review
- Small RNA-Omics for Plant Virus Identification, Virome Reconstruction, and Antiviral Defense Characterization.Frontiers in microbiology · 2018Review
- New DRB complexes for new DRB functions in plants.RNA biology · 2017Article
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Authors and funding
10 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Small RNAs play essential regulatory roles in genome stability, development, and responses to biotic and abiotic stresses in most eukaryotes. In plants, the RNaseIII enzyme DICER-LIKE1 (DCL1) produces miRNAs, whereas DCL2, DCL3, and DCL4 produce various size classes of siRNAs. Plants also encode RNASE THREE-LIKE (RTL) enzymes that lack DCL-specific domains and whose function is largely unknown. We found that virus infection induces RTL1 expression, suggesting that this enzyme could play a role in plant-virus interaction. To first investigate the biochemical activity of RTL1 independent of virus infection, small RNAs were sequenced from transgenic plants constitutively expressing RTL1. These plants lacked almost all DCL2-, DCL3-, and DCL4-dependent small RNAs, indicating that RTL1 is a general suppressor of plant siRNA pathways. In vivo and in vitro assays revealed that RTL1 prevents siRNA production by cleaving dsRNA prior to DCL2-, DCL3-, and DCL4-processing. The substrate of RTL1 cleavage is likely long-perfect (or near-perfect) dsRNA, consistent with the RTL1-insensitivity of miRNAs, which derive from DCL1-processing of short-imperfect dsRNA. Virus infection induces RTL1 mRNA accumulation, but viral proteins that suppress RNA silencing inhibit RTL1 activity, suggesting that RTL1 has evolved as an inducible antiviral defense that could target dsRNA intermediates of viral replication, but that a broad range of viruses counteract RTL1 using the same protein toolbox used to inhibit antiviral RNA silencing. Together, these results reveal yet another level of complexity in the evolutionary battle between viruses and plant defenses.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.