Evidence map›Paper›PMID 26694837›Full record

ArticleThe Journal of cell biology2015

In vivo single-particle imaging of nuclear mRNA export in budding yeast demonstrates an essential role for Mex67p.

Carlas Smith, Azra Lari, Carina Patrizia Derrer, Anette Ouwehand, Ammeret Rossouw, Maximiliaan Huisman, Thomas Dange, Mark Hopman, Aviva Joseph, Daniel Zenklusen and 3 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of cell biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.

0numbers the graph read from it
0cells of the map it votes in
40citing papers in PubMed
5.6field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

40 citing papers in PubMed, 82 citations in OpenAlex.

  1. Review
  2. Article
  3. Structure, function and assembly of nuclear pore complexes.Nature reviews. Molecular cell biology · 2026
    Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. The Great Escape: mRNA Export through the Nuclear Pore Complex.International journal of molecular sciences · 2021
    Review
  9. Review
  10. Review
  11. Article
  12. Article
  13. Article
  14. Article
  15. Mapping the native organization of the yeast nuclear pore complex using nuclear radial intensity measurements.Proceedings of the National Academy of Sciences of the United States of America · 2019
    Article
  16. Review
  17. Article
  18. Live-Cell Imaging of mRNP-NPC Interactions in Budding Yeast.Methods in molecular biology (Clifton, N.J.) · 2019
    Article
  19. Article
  20. Identification of the NovelG3 (Bethesda, Md.) · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 5 institutions in 4 countries.

Carlas SmithRNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA 01605 Department Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605.
Azra LariDepartment of Cell Biology, University of Alberta, T6G 2H7 Edmonton, Alberta, Canada.
Carina Patrizia DerrerDepartment of Biology, Institute of Biochemistry, ETH Zurich, 8093 Zurich, Switzerland.
Anette OuwehandRNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA 01605 Department Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605 Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, 2628 CJ Delft, Netherlands.
Ammeret RossouwRNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA 01605 Department Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605 Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, 2628 CJ Delft, Netherlands.
Maximiliaan HuismanRNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA 01605 Department Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605 Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, 2628 CJ Delft, Netherlands.
Thomas DangeBionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, 2628 CJ Delft, Netherlands.
Mark HopmanBionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, 2628 CJ Delft, Netherlands.
Aviva JosephRNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA 01605 Department Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605.
Daniel ZenklusenDepartement de Biochimie et Medecine Moleculaire, Universite de Montreal, H3T 1J4 Montreal, Quebec, Canada.
Karsten WeisDepartment of Biology, Institute of Biochemistry, ETH Zurich, 8093 Zurich, Switzerland Department of Cell and Developmental Biology, University of California, Berkeley, Berkeley, CA 94720 ben.montpetit@ualberta.ca david.grunwald@umassmed.edu karsten.weis@bc.biol.ethz.ch.
David GrunwaldRNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA 01605 Department Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605 Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, 2628 CJ Delft, Netherlands ben.montpetit@ualberta.ca david.grunwald@umassmed.edu karsten.weis@bc.biol.ethz.ch.
Ben MontpetitDepartment of Cell Biology, University of Alberta, T6G 2H7 Edmonton, Alberta, Canada ben.montpetit@ualberta.ca david.grunwald@umassmed.edu karsten.weis@bc.biol.ethz.ch.
Delft University of Technology · NLUniversity of Massachusetts Chan Medical School · USETH Zurich · CHUniversity of Alberta · CAUniversité de Montréal · CA

Funding

X-ray Cryst. CoreP50GM082545 · NIGMS · UNIVERSITY OF UTAH · PI SUNDQUIST, WESLEY I. · 2007 to 2018
$51.1M
RNA TRANSPORT AND LOCALIZATION IN YEAST IN SITUR01GM057071 · NIGMS · YESHIVA UNIVERSITY · PI SINGER, ROBERT H · 1998 to 2019
$5.7M
Structure and function of the nuclear pore complexR01GM058065 · NIGMS · UNIVERSITY OF CALIFORNIA SAN FRANCISCO · PI HEALD, REBECCA W · 1998 to 2015
$4.6M
CIHR MOP130231Howard Hughes Medical InstituteNIGMS NIH HHS GM058065NIGMS NIH HHS GM57071NIGMS NIH HHS R01 GM057071NIGMS NIH HHS R01 GM058065
6 · The paper itself

Abstract

Many messenger RNA export proteins have been identified; yet the spatial and temporal activities of these proteins and how they determine directionality of messenger ribonucleoprotein (mRNP) complex export from the nucleus remain largely undefined. Here, the bacteriophage PP7 RNA-labeling system was used in Saccharomyces cerevisiae to follow single-particle mRNP export events with high spatial precision and temporal resolution. These data reveal that mRNP export, consisting of nuclear docking, transport, and cytoplasmic release from a nuclear pore complex (NPC), is fast (∼ 200 ms) and that upon arrival in the cytoplasm, mRNPs are frequently confined near the nuclear envelope. Mex67p functions as the principal mRNP export receptor in budding yeast. In a mex67-5 mutant, delayed cytoplasmic release from NPCs and retrograde transport of mRNPs was observed. This proves an essential role for Mex67p in cytoplasmic mRNP release and directionality of transport.

Indexed as

RNA TransportCell NucleusNuclear ProteinsNucleocytoplasmic Transport ProteinsRNA-Binding ProteinsRNA, FungalRNA, MessengerSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsMEX67 protein, S cerevisiaeNuclear ProteinsNucleocytoplasmic Transport ProteinsRNA-Binding ProteinsRNA, FungalRNA, MessengerSaccharomyces cerevisiae Proteins

Identifiers

PMID26694837
PMCPMC4687877
OpenAlexW2201364792

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.