ArticleViruses2015
Rapid Detection of Listeria by Bacteriophage Amplification and SERS-Lateral Flow Immunochromatography.
Article in Viruses, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- A Critical Analysis of the Opportunities and Challenges of Phage Application in Seafood Quality Control.Foods (Basel, Switzerland) · 2024Review
- Advanced Vibrational Spectroscopy and Bacteriophages Team Up: Dynamic Synergy for Medical and Environmental Applications.International journal of molecular sciences · 2024Review
- Surface-enhanced Raman spectroscopy for the characterization of bacterial pellets ofRSC advances · 2024Article
- Advances in detection methods for viable Salmonella spp.: current applications and challenges.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Review
- Development and Evaluation of Fluorescence Immunochromatography for Rapid and Sensitive Detection of ThermophilicFood safety (Tokyo, Japan) · 2021Article
- Antibody- and nucleic acid-based lateral flow immunoassay for Listeria monocytogenes detection.Analytical and bioanalytical chemistry · 2021Review
- Automatic and sensitive detection of West Nile virus non-structural protein 1 with a portable SERS-LFIA detector.Mikrochimica acta · 2021Article
- The Application of Bacteriophage Diagnostics for Bacterial Pathogens in the Agricultural Supply Chain: From Farm-to-Fork.PHAGE (New Rochelle, N.Y.) · 2020Review
- Surface-Enhanced Raman Scattering-Based Lateral-Flow Immunoassay.Nanomaterials (Basel, Switzerland) · 2020Review
- Reporter Phage-Based Detection of Bacterial Pathogens: Design Guidelines and Recent Developments.Viruses · 2020Review
- Immunomagnetic separation and Listeriamonocytogenes detection with surface-enhanced Raman scatteringTurkish journal of medical sciences · 2020Article
- Methods for detection of viable foodborne pathogens: current state-of-art and future prospects.Applied microbiology and biotechnology · 2020Review
- Lab-Made Electronic Nose for Fast Detection ofVeterinary sciences · 2020Article
- Article
- Strain-level typing and identification of bacteria - a novel approach for SERS active plasmonic nanostructures.Analytical and bioanalytical chemistry · 2018Article
- Dual dye-loaded Au@Ag coupled to a lateral flow immunoassay for the accurate and sensitive detection ofRSC advances · 2018Article
- Characterization and Discrimination of Gram-Positive Bacteria Using Raman Spectroscopy with the Aid of Principal Component Analysis.Nanomaterials (Basel, Switzerland) · 2017Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A rapid Listeria detection method was developed utilizing A511 bacteriophage amplification combined with surface-enhanced Raman spectroscopy (SERS) and lateral flow immunochromatography (LFI). Anti-A511 antibodies were covalently linked to SERS nanoparticles and printed onto nitrocellulose membranes. Antibody-conjugated SERS nanoparticles were used as quantifiable reporters. In the presence of A511, phage-SERS nanoparticle complexes were arrested and concentrated as a visible test line, which was interrogated quantitatively by Raman spectroscopy. An increase in SERS intensity correlated to an increase in captured phage-reporter complexes. SERS limit of detection was 6 × 10(6) pfu·mL(-1), offering detection below that obtainable by the naked eye (LOD 6 × 10(7) pfu·mL(-1)). Phage amplification experiments were carried out at a multiplicity of infection (MOI) of 0.1 with 4 different starting phage concentrations monitored over time using SERS-LFI and validated by spot titer assay. Detection of L. monocytogenes concentrations of 1 × 10(7) colony forming units (cfu)·mL(-1), 5 × 10(6) cfu·mL(-1), 5 × 10(5) cfu·mL(-1) and 5 × 10(4) cfu·mL(-1) was achieved in 2, 2, 6, and 8 h, respectively. Similar experiments were conducted at a constant starting phage concentration (5 × 10(5) pfu·mL(-1)) with MOIs of 1, 2.5, and 5 and were detected in 2, 4, and 5 h, respectively.
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