ArticleMolecular bioSystems2016
Neurospora crassa transcriptomics reveals oxidative stress and plasma membrane homeostasis biology genes as key targets in response to chitosan.
Article in Molecular bioSystems, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 38 citations in OpenAlex.
- Staurosporine as an Antifungal Agent.International journal of molecular sciences · 2025Review
- Chitosan and its derivatives regulate lactic acid synthesis during milk fermentation.Frontiers in nutrition · 2024Article
- Low Molecular Weight and High Deacetylation Degree Chitosan Batch Alleviates Pathogenesis, Toxin Accumulation, andInternational journal of molecular sciences · 2023Article
- Chitosan: An Autocidal Molecule of Plant Pathogenic Fungus.Life (Basel, Switzerland) · 2022Review
- Strain Degeneration inJournal of fungi (Basel, Switzerland) · 2021Article
- Chitosan modulates Pochonia chlamydosporia gene expression during nematode egg parasitism.Environmental microbiology · 2021Article
- Antimicrobial Actions and Applications of Chitosan.Polymers · 2021Review
- Molecular Mechanisms of Chitosan Interactions with Fungi and Plants.International journal of molecular sciences · 2019Review
- Identification and manipulation ofBiotechnology for biofuels · 2019Article
- Response of Ustilago maydis against the Stress Caused by Three Polycationic Chitin Derivatives.Molecules (Basel, Switzerland) · 2017Article
- Regulated Forms of Cell Death in Fungi.Frontiers in microbiology · 2017Review
- Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Chitosan is a natural polymer with antimicrobial activity. Chitosan causes plasma membrane permeabilization and induction of intracellular reactive oxygen species (ROS) in Neurospora crassa. We have determined the transcriptional profile of N. crassa to chitosan and identified the main gene targets involved in the cellular response to this compound. Global network analyses showed membrane, transport and oxidoreductase activity as key nodes affected by chitosan. Activation of oxidative metabolism indicates the importance of ROS and cell energy together with plasma membrane homeostasis in N. crassa response to chitosan. Deletion strain analysis of chitosan susceptibility pointed NCU03639 encoding a class 3 lipase, involved in plasma membrane repair by lipid replacement, and NCU04537 a MFS monosaccharide transporter related to assimilation of simple sugars, as main gene targets of chitosan. NCU10521, a glutathione S-transferase-4 involved in the generation of reducing power for scavenging intracellular ROS is also a determinant chitosan gene target. Ca(2+) increased tolerance to chitosan in N. crassa. Growth of NCU10610 (fig 1 domain) and SYT1 (a synaptotagmin) deletion strains was significantly increased by Ca(2+) in the presence of chitosan. Both genes play a determinant role in N. crassa membrane homeostasis. Our results are of paramount importance for developing chitosan as an antifungal.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.