ArticleMeta gene2016
RAPD and ISSR based evaluation of genetic stability of micropropagated plantlets of Morus alba L. variety S-1.
Article in Meta gene, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Induction of Axillary Bud Swelling ofPlants (Basel, Switzerland) · 2023Article
- Biotechnological Advances in Pharmacognosy and In Vitro Manipulation ofPlants (Basel, Switzerland) · 2022Review
- Cytokinin-Based Tissue Cultures for Stable Medicinal Plant Production: Regeneration and Phytochemical Profiling ofBiomolecules · 2021Article
- Applications of Cytokinins in Horticultural Fruit Crops: Trends and Future Prospects.Biomolecules · 2020Review
- In Vitro Propagation of an EndangeredPlants (Basel, Switzerland) · 2020Article
- Indirect regeneration and genetic fidelity analysis of acclimated plantlets through SCoT and ISSR markers inBiotechnology reports (Amsterdam, Netherlands) · 2020Article
- Rapid plant regeneration in industrially important3 Biotech · 2020Article
- Genetic homogeneity revealed in micropropagatedPhysiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Thidiazuron (TDZ) induced organogenesis and clonal fidelity studies inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2018Article
- Evaluation of genetic relationship between 15 bamboo species of North-East India based on ISSR marker analysis.Molecular biology research communications · 2018Article
- Improvement of abiotic stress adaptive traits in mulberry (Morus spp.): an update on biotechnological interventions.3 Biotech · 2017Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant regeneration through rapid in vitro clonal propagation of nodal explants of Morus alba L. variety S-1 was established along with genetic stability analysis of regenerates. Axillary shoot bud proliferation was achieved on Murashige and Skoog (MS) medium in various culture regimes. Highest number of shoots (5.62 ± 0.01), with average length 4.19 ± 0.01 cm, was initially achieved with medium containing 0.5 mg/l N(6)-benzyladenine (BA) and 3% sucrose. Repeated subculturing of newly formed nodal parts after each harvest up to sixth passage, yielded highest number of shoots (about 32.27) per explants was obtained after fourth passage. Rooting of shoots occurred on 1/2 MS medium supplemented with 1.0 mg/1 Indole-3-butyric acid (IBA). About 90% (89.16) of the plantlets transferred to the mixture of sand:soil:organic manure (2:2:1) in small plastic pots acclimatized successfully. Genetic stability of the discussed protocol was confirmed by two DNA-based fingerprinting techniques i.e. RAPD (random amplified polymorphic DNA) and ISSR (inter-simple sequence repeat). This protocol can be used for commercial propagation and for future genetic improvement studies.
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