ArticleBMC physiology2015
An investigation of the efficacy and mechanism of contrast-enhanced X-ray computed tomography utilizing iodine for large specimens through experimental and simulation approaches.
Article in BMC physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 46 citations in OpenAlex.
- Syrinx anatomy and vocalizations in New World vultures (Aves: Cathartidae): Implications for avian vocal evolution.Journal of anatomy · 2026Article
- Behavioral correlates of fascicular organization: The confluence of muscle architectural anatomy and function.Anatomical record (Hoboken, N.J. : 2007) · 2025Review
- Comparing microCT Staining and Scanning Methodology for Brain Studies in Various Sizes of Spiders.The Journal of comparative neurology · 2025Article
- Spatial and temporal evaluation of iodine uptake and radiodensity in meniscus tissue using contrast-enhanced micro-CT.Heliyon · 2024Article
- Evolution of the syrinx of Apodiformes, including the vocal-learning Trochilidae (Aves: Strisores).Zoological journal of the Linnean Society · 2024Article
- Experimental assessment of diffusible iodine-based contrast-enhanced computed tomography (diceCT) protocols.PeerJ · 2024Article
- Iodine-enhanced micro-computed tomography of atherosclerotic plaque morphology complements conventional histology.Atherosclerosis · 2020Article
- Iodine concentration and content measured by dual-source computed tomography are correlated to thyroid hormone levels in euthyroid patients: a cross-sectional study in China.BMC medical imaging · 2020Article
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- Application of custom anatomy-based nerve conduits on rabbit sciatic nerve defects:Neural regeneration research · 2019Article
- Micro-computed tomography: a new diagnostic tool in postmortem assessment of brain anatomy in small fetuses.Neuroradiology · 2019Article
- Iodine-Enhanced Micro-CT Imaging of Soft Tissue on the Example of Peripheral Nerve Regeneration.Contrast media & molecular imaging · 2019Article
- 3D Muscle Architecture of the Pectoral Muscles of European Starling (Integrative organismal biology (Oxford, England) · 2019Article
- Dynamic Musculoskeletal Functional Morphology: Integrating diceCT and XROMM.Anatomical record (Hoboken, N.J. : 2007) · 2018Article
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- Iodine concentration calculated by dual-energy computed tomography (DECT) as a functional parameter to evaluate thyroid metabolism in patients with hyperthyroidism.BMC medical imaging · 2017Article
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- Micro-computed tomography in murine models of cerebral cavernous malformations as a paradigm for brain disease.Journal of neuroscience methods · 2016Article
- Article
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5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIodine-based solutions have long been known to be effective in aiding the differentiation among soft tissues in both fundamental anatomical research and for clinical diagnoses. Recently the combination of this particular contrasting agent with micro-computed tomography (micro-CT) has resulted in an array of high-quality image data, in which anatomical structures not visible in conventional CT can be identified and quantified. However, there has been only limited data available to inform detailed protocols for staining large specimens. Further, modeling of the staining mechanism has focused on simple diffusion processes.
resultsA low concentration of iodine-based buffered formalin solution with a long staining period was used to visualize soft-tissue structures in a large goose head. The staining effect was analyzed by serially measuring the micro-CT profiles across coronal sections throughout the staining period. Regular replacement of the staining solution combined with a longer staining period significantly improved contrast within tissues. A simplified one-dimensional Diffusion-Sorption model with a three-zone domain was used to simulate the diffusion process by calculating the concentration profile of iodine across the adductor region, which fits well with the experiment data. Observations of changes in the concentration of the staining agent and simulation results suggest that the sorption of iodine by tissues significantly affects the effective diffusion coefficient for the contrasting agent.
conclusionsThe Diffusion-Sorption model better explains previously reported difficulties in staining large samples comprised of tissues with high partition coefficients (K d ). Differences in partition coefficient (K d ), bulk density (ρ b ), and porosity (θ) could further explain the observed variation in staining rate and maximal staining effect among different tissues. Recommended protocols for staining large specimens are detailed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.