ArticleScientific reports2015
Directly interrogating single quantum dot labelled UvrA2 molecules on DNA tightropes using an optically trapped nanoprobe.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed.
- Double vision: 2D and 3D mosquito trajectories can be as valuable for behaviour analysis via machine learning.Parasites & vectors · 2024Article
- Single-molecule imaging reveals the concerted release of myosin from regulated thin filaments.eLife · 2021Article
- Replication of the Mammalian Genome by Replisomes Specific for Euchromatin and Heterochromatin.Frontiers in cell and developmental biology · 2021Article
- Single-Cell Elasticity Measurement with an Optically Actuated Microrobot.Micromachines · 2020Article
- Multiview microscopy of single cells through microstructure-based indirect optical manipulation.Biomedical optics express · 2020Article
- Indirect optical trapping using light driven micro-rotors for reconfigurable hydrodynamic manipulation.Nature communications · 2019Article
- Chromatin architecture changes and DNA replication fork collapse are critical features in cryopreserved cells that are differentially controlled by cryoprotectants.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
In this study we describe a new methodology to physically probe individual complexes formed between proteins and DNA. By combining nanoscale, high speed physical force measurement with sensitive fluorescence imaging we investigate the complex formed between the prokaryotic DNA repair protein UvrA2 and DNA. This approach uses a triangular, optically-trapped "nanoprobe" with a nanometer scale tip protruding from one vertex. By scanning this tip along a single DNA strand suspended between surface-bound micron-scale beads, quantum-dot tagged UvrA2 molecules bound to these '"DNA tightropes" can be mechanically interrogated. Encounters with UvrA2 led to deflections of the whole nanoprobe structure, which were converted to resistive force. A force histogram from all 144 detected interactions generated a bimodal distribution centered on 2.6 and 8.1 pN, possibly reflecting the asymmetry of UvrA2's binding to DNA. These observations successfully demonstrate the use of a highly controllable purpose-designed and built synthetic nanoprobe combined with fluorescence imaging to study protein-DNA interactions at the single molecule level.
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