ArticlePloS one2015
Monitoring Astrocytic Proteome Dynamics by Cell Type-Specific Protein Labeling.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Astrocytes viewed through the lens of their proteomes and subproteomes.Nature reviews. Neuroscience · 2026Review
- Acid Cleavable Biotin-Alkyne Improves Sensitivity for Direct Detection of Biotin Labeled Peptides in Biorthogonal Noncanonical Amino Acid Tagging Analysis.Journal of proteome research · 2024Article
- Acid cleavable biotin-alkyne improves sensitivity for direct detection of biotin labeled peptides in BONCAT analysis.bioRxiv : the preprint server for biology · 2024Article
- Manipulation of DHPS activity affects dendritic morphology and expression of synaptic proteins in primary rat cortical neurons.Frontiers in cellular neuroscience · 2024Article
- Deoxynivalenol affects cell metabolism in vivo and inhibits protein synthesis in IPEC-1 cells.Mycotoxin research · 2023Article
- Cell-selective proteomics segregates pancreatic cancer subtypes by extracellular proteins in tumors and circulation.Nature communications · 2023Article
- A novel method for visualizingiScience · 2023Article
- Activity-dependent translation dynamically alters the proteome of the perisynaptic astrocyte process.Cell reports · 2022Article
- Secondary thalamic neuroinflammation after focal cortical stroke and traumatic injury mirrors corticothalamic functional connectivity.The Journal of comparative neurology · 2022Article
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- Click Chemistry (CuAAC) and Detection of TaggedBio-protocol · 2019Article
- Non-canonical amino acid labeling in proteomics and biotechnology.Journal of biological engineering · 2019Review
- Article
- Application of bio-orthogonal proteome labeling to cell transplantation and heterochronic parabiosis.Nature communications · 2017Article
- Tissue Specific Labeling in Proteomics.Proteomes · 2017Review
- Cell-selective proteomics for biological discovery.Current opinion in chemical biology · 2017Review
- Mapping Gene Expression in Excitatory Neurons during Hippocampal Late-Phase Long-Term Potentiation.Frontiers in molecular neuroscience · 2017Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability of the nervous system to undergo long-term plasticity is based on changes in cellular and synaptic proteomes. While many studies have explored dynamic alterations in neuronal proteomes during plasticity, there has been less attention paid to the astrocytic counterpart. Indeed, progress in identifying cell type-specific proteomes is limited owing to technical difficulties. Here, we present a cell type-specific metabolic tagging technique for a mammalian coculture model based on the bioorthogonal amino acid azidonorleucine and the mutated Mus musculus methionyl-tRNA synthetaseL274G enabling azidonorleucine introduction into de novo synthesized proteins. Azidonorleucine incorporation resulted in cell type-specific protein labeling and retained neuronal or astrocytic cell viability. Furthermore, we were able to label astrocytic de novo synthesized proteins and identified both Connexin-43 and 60S ribosomal protein L10a upregulated upon treatment with Brain-derived neurotrophic factor in astrocytes of a neuron-glia coculture. Taken together, we demonstrate the successful dissociation of astrocytic from neuronal proteomes by cell type-specific metabolic labeling offering new possibilities for the analyses of cell type-specific proteome dynamics.
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