ArticleJournal of genetics2015
Chalcone synthase genes from milk thistle (Silybum marianum): isolation and expression analysis.
Article in Journal of genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 30 citations in OpenAlex.
- Silver nanoparticles enhance organogenesis, total flavonoid and anthocyanin contents inBiotechnology reports (Amsterdam, Netherlands) · 2026Article
- Effect of multi-walled carbon nanotube exposure on the expression pattern of genes involved in silymarin synthesis pathway in Silybum Marianum L.BMC plant biology · 2025Article
- Nanoparticle-boosted silymarin: Are we overlooking taxifolin and other key components?Frontiers in plant science · 2025Article
- Genomic and Expression Analysis of Cassava (Genes · 2024Article
- Article
- Foliar spraying with zinc oxide nanoparticles enhances the anti-osteoporotic efficacy of the fruit extracts ofFrontiers in plant science · 2024Article
- Bioconversion of Callus-Produced Precursors to Silymarin Derivatives inInternational journal of molecular sciences · 2021Article
- The Impact of Drought Stress on Antioxidant Responses and Accumulation of Flavonolignans in Milk Thistle (Plants (Basel, Switzerland) · 2019Article
- Functional study of CHS gene family members in citrus revealed a novel CHS gene affecting the production of flavonoids.BMC plant biology · 2018Article
- Chemotaxonomic and biosynthetic relationships between flavonolignans produced by Silybum marianum populations.Fitoterapia · 2017Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Silymarin is a flavonoid compound derived from milk thistle (Silybum marianum) seeds which has several pharmacological applications. Chalcone synthase (CHS) is a key enzyme in the biosynthesis of flavonoids; thereby, the identification of CHS encoding genes in milk thistle plant can be of great importance. In the current research, fragments of CHS genes were amplified using degenerate primers based on the conserved parts of Asteraceae CHS genes, and then cloned and sequenced. Analysis of the resultant nucleotide and deduced amino acid sequences led to the identification of two different members of CHS gene family,SmCHS1 and SmCHS2. Third member, full-length cDNA (SmCHS3) was isolated by rapid amplification of cDNA ends (RACE), whose open reading frame contained 1239 bp including exon 1 (190 bp) and exon 2 (1049 bp), encoding 63 and 349 amino acids, respectively. In silico analysis of SmCHS3 sequence contains all the conserved CHS sites and shares high homology with CHS proteins from other plants.Real-time PCR analysis indicated that SmCHS1 and SmCHS3 had the highest transcript level in petals in the early flowering stage and in the stem of five upper leaves, followed by five upper leaves in the mid-flowering stage which are most probably involved in anthocyanin and silymarin biosynthesis.
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