ArticleMethods (San Diego, Calif.)2016
Quantitative spatial analysis of transcripts in multinucleate cells using single-molecule FISH.
Article in Methods (San Diego, Calif.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- Sequence and structure of protein binding sites in RNA impact biomolecular condensates.Molecular biology of the cell · 2026Article
- Post-transcriptional regulatory networks: The dynamic interplay of RNA-binding proteins.The FEBS journal · 2026Review
- Modern RNA Quantification Methods: From RT-qPCR to Advanced Microscopy.The journal of physical chemistry. B · 2026Review
- Sequence and structure of protein binding sites in RNA impact biomolecular condensates.bioRxiv : the preprint server for biology · 2026Article
- RNA encodes physical information.bioRxiv : the preprint server for biology · 2024Article
- Spatial multi-omics: deciphering technological landscape of integration of multi-omics and its applications.Journal of hematology & oncology · 2024Review
- Intrinsically disordered sequences can tune fungal growth and the cell cycle for specific temperatures.Current biology : CB · 2024Article
- Single-Molecule Fluorescent In Situ Hybridization (smFISH) for RNA Detection in the Fungal Pathogen Candida albicans.Methods in molecular biology (Clifton, N.J.) · 2024Article
- PRD-2 mediates clock-regulated perinuclear localization of clock gene RNAs within the circadian cycle ofProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Interchromosomal interaction of homologous Stat92E alleles regulates transcriptional switch during stem-cell differentiation.Nature communications · 2022Article
- Image-Based Single-Molecule Analysis of Notch-Dependent Transcription in Its Natural Context.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Quantitative single molecule RNA-FISH and RNase-free cell wall digestion in Neurospora crassa.Fungal genetics and biology : FG & B · 2021Article
- Long QT SyndromeCirculation. Arrhythmia and electrophysiology · 2021Article
- Three-dimensional Reconstruction and Quantification of Proteins and mRNAs at the Single-cell Level in Cultured Cells.Bio-protocol · 2019Article
- Single-molecule FluorescenceBio-protocol · 2018Article
- Single-molecule RNA FluorescenceBio-protocol · 2017Article
- Article
- Clustered nuclei maintain autonomy and nucleocytoplasmic ratio control in a syncytium.Molecular biology of the cell · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
mRNA positioning in the cell is important for diverse cellular functions and proper development of multicellular organisms. Single-molecule RNA FISH (smFISH) enables quantitative investigation of mRNA localization and abundance at the level of individual molecules in the context of cellular features. Details about spatial mRNA patterning at various times, in different genetic backgrounds, at different developmental stages, and under varied environmental conditions provide invaluable insights into the mechanisms and functions of spatial regulation. Here, we describe detailed methods for performing smFISH along with immunofluorescence for two large, multinucleate cell types: the fungus Ashbya gossypii and cultured mouse myotubes. We also put forward a semi-automated image processing tool that systematically detects mRNAs from smFISH data and statistically analyzes the spatial pattern of mRNAs using a customized MATLAB code. These protocols and image analysis tools can be adapted to a wide variety of transcripts and cell types for systematically and quantitatively analyzing mRNA distribution in three-dimensional space.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.