ArticleStem cell research & therapy2015
Combining mesenchymal stem cell sheets with platelet-rich plasma gel/calcium phosphate particles: a novel strategy to promote bone regeneration.
Article in Stem cell research & therapy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 64 citations in OpenAlex.
- Platelet-rich plasma for the treatment of bone defects: from pre-clinical rational to evidence in the clinical practice. A systematic review.International orthopaedics · 2017Pooled it
- CGRP-empowered stem cell sheet/short nanofiber spongeMaterials today. Bio · 2026Article
- Article
- Novel platelet-rich plasma/ hyaluronic acid lyophilized formulations for wound healing applications.Frontiers in bioengineering and biotechnology · 2025Article
- The Clinical Application of Gel-Based Composite Scaffolds in Rotator Cuff Repair.Gels (Basel, Switzerland) · 2024Review
- Plasma Rich in Growth Factors in Bone Regeneration: The Proximity to the Clot as a Differential Factor in Osteoblast Cell Behaviour.Dentistry journal · 2024Article
- Dual aldehyde cross-linked hyaluronic acid hydrogels loaded with PRP and NGF biofunctionalized PEEK interfaces to enhance osteogenesis and vascularization.Materials today. Bio · 2024Article
- A Comprehensive Review of Platelet-Rich Plasma and Its Emerging Role in Accelerating Bone Healing.Cureus · 2024Review
- Advances with Platelet-Rich Plasma for Bone Healing.Biologics : targets & therapy · 2024Review
- Arthroscopic Treatment of a Subchondral Bone Cyst via Stem Cells Application: A Case Study in Equine Model and Outcomes.Biomedicines · 2023Article
- Application of mesenchymal stem cell sheet for regeneration of craniomaxillofacial bone defects.Stem cell research & therapy · 2023Review
- Construction of biomimetic cell-sheet-engineered periosteum with a double cell sheet to repair calvarial defects of rats.Journal of orthopaedic translation · 2023Article
- The Combination of Platelet Rich Plasma Gel, Human Umbilical Mesenchymal Stem Cells and Nanohydroxyapatite/polyamide 66 Promotes Angiogenesis and Bone Regeneration in Large Bone Defect.Tissue engineering and regenerative medicine · 2022Article
- The effect of LyPRP/collagen composite hydrogel on osteogenic differentiation of rBMSCs.Regenerative biomaterials · 2021Article
- A novel tissue-engineered bone graft composed of silicon-substituted calcium phosphate, autogenous fine particulate bone powder and BMSCs promotes posterolateral spinal fusion in rabbits.Journal of orthopaedic translation · 2021Article
- Autogenous bone particles combined with platelet-rich plasma can stimulate bone regeneration in rabbits.Experimental and therapeutic medicine · 2020Article
- The platelet-rich plasma and mesenchymal stem cell milieu: A review of therapeutic effects on bone healing.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2020Review
- CircFOXP1/FOXP1 promotes osteogenic differentiation in adipose-derived mesenchymal stem cells and bone regeneration in osteoporosis via miR-33a-5p.Journal of cellular and molecular medicine · 2020Article
- Coaxial nanofiber scaffold with super-active platelet lysate to accelerate the repair of bone defects.RSC advances · 2020Article
- Platelet-rich plasma-enhanced osseointegration of decellularized bone matrix in critical-size radial defects in rabbits.Annals of translational medicine · 2020Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPromotion of bone regeneration is important for successful repair of bony defects. This study aimed to investigate whether combining bone marrow-derived mesenchymal stem cell (BMSC) sheets with platelet-rich plasma (PRP) gel/calcium phosphate particles could promote bone formation in the femoral bone defects of rats.
methodsThe proliferation and differentiation of BMSCs or BMSC sheets cultured with calcium phosphate particles and/or PRP were investigated in in vitro. In vivo, 36 2.5 × 5 mm bone defects were randomly divided into groups and treated with either BMSCs/PRP gel, calcium phosphate particles, PRP gel/calcium phosphate particles, a BMSC sheet/calcium phosphate particles, a BMSC sheet/PRP gel/calcium phosphate particles, or were left untreated (n = 6/group). A further 15 bone defects were treated with chloromethyl-benzamidodialkylcarbocyanine (CM-Dil)-labelled BMSC sheet/PRP gel/calcium phosphate particles and observed using a small animal in vivo fluorescence imaging system to trace the implanted BMSCs at 1 day, 3 days, 7 days, 2 weeks, and 4 weeks after surgery.
resultsThe expression of collagen type I and osteocalcin genes of BMSCs or BMSC sheets treated with PRP and calcium phosphate particles was significantly higher than that of BMSCs or BMSC sheets treated with calcium phosphate particles or the controls (P <0.05). PRP can promote gene expression of collagen III and tenomodulin by BMSCs and in BMSC sheets. The VEGF, collagen I and osteocalcin gene expression levels were higher in the BMSC sheet than in cultured BMSCs (P <0.05). Moreover, alizarin red staining quantification, ALP quantification and calcein blue fluorescence showed the osteogenic potential of BMSCs treated with PRP and calcium phosphate particles The implanted BMSCs were detectable at 1 day, 3 days, 7 days, 2 weeks and 4 weeks after surgery by a small animal in vivo fluorescence imaging system and were visualized in the defect zones by confocal microscopy. At 4 weeks after implantation, the defects treated with the BMSC sheet/PRP gel/calcium phosphate particles showed significantly more bone formation than the other five groups.
conclusionsIncorporation of an BMSC sheet into the PRP gel/calcium phosphate particles greatly promoted bone regeneration. These BMSC sheet and tissue engineering strategies offer therapeutic opportunities for promoting bone defect repair clinically.
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